摘要
目的了解无锡市HIV-1亚型流行及进化特征,为预测本地HIV-1疫情变化提供参考依据。方法样本来源于2013年4月至2016年7月无锡市部分CD4+T淋巴细胞监测队列,进行HIV-1基因的扩增和测序,采用ChromasPro 1.6和MEGA 7.0软件构建HIV-1序列数据库;采用FastTree 2.1.10和BEAST 1.7.2软件和贝叶斯系统进化推断法重构HIV-1历史传播情况,采用SPSS 22.0软件进行统计学分析。结果有205例HIV-1感染者,其中≥50岁占32.68%(67/205)。共检测出CRF01_AE、CRF07_BC、CRF67_01B、B、CRF08_BC、CRF68_0B、CRF78_cpx 7种HIV-1基因型及1例独特重组型。流行亚型以CRF01_AE(51.67%,93/180)及CRF07_BC(17.22%,31/180)为主,不同亚型之间传播方式的差异有统计学意义(χ2=16.99,P≤0.05)。CRF67_01B型(12.78%,23/180)所占比例较高。贝叶斯系统进化推断法分析结果显示,无锡市CRF67_01B型进化率为2.29×10-3,最近共同祖先时间约为2003.10年,与江苏省及安徽省来源的参考株可能存在亲缘关系,CRF67_01B型于2003年开始在无锡市出现传播。结论2013-2016年无锡市HIV-1亚型复杂多样,CRF67_01B型已经开始在无锡市流行,应持续监测HIV-1亚型变化,从分子角度为疫情预测提供参考依据。
Objective To understand the distribution of HIV-1 subtype in Wuxi city,to predict the local HIV-1 epidemics.Methods Samples were collected from the'CD4+T lymphocyte monitoring cohort study’in Wuxi from April 2013 to July 2016.HIV-1 gene was amplified,sequenced and with HIV-1 sequence database constructed,using both the ChromasPro 1.6 and MEGA 7.0 softwares.Bayesian phylogenetic inference was used to rebuild the history of HIV-1 transmission,while BEAST 1.7.2 and FastTree 2.1.10 software were used for data analysis.Statistical analysis using SPSS 22.0 software.Results Among the 205 subjects of HIV-1 infection,32.68%(67/205)of them were over 50 years old.Seven subtypes(including CRF01_AE,CRF07_BC,CRF67_01B,B,CRF08_BC,CRF68_0B,CRF78_cpx)and one of the unique recombinant forms(URFs)were detected.The main subtypes were CRF01_AE(51.67%,93/180)and CRF07_BC(17.22%,31/180).Differences between subtypes and ways of transmission were statistically significant(χ2=16.99,P≤0.05).The proportion of CRF67_01B(12.78%,23/180)was higher than before.Results from Bayesian phylogenetic inference analysis showed that the evolution rate was 2.29×10-3 and Time to the Most Recent Common Ancestor(tMRCA)was 2003.10.CRF67_01B was probably related to the reference strains from Jiangsu and Anhui provinces,and had been spreading in Wuxi since 2003.Conclusions HIV-1 subtypes seemed complex and diverse in Wuxi city in 2013-2016,with CRF67_01B being pervasive.Continuous molecular monitor program was still needed to provide reference for the prediction of epidemics,from the molecular perspective.
作者
殷玥琪
陈剑双
成浩
张轩
吴楠楠
陈璐斯
王蓓
Yin Yueqi;Chen Jianshuang;Cheng Hao;Zhang Xuan;Wu Nannan;Chen Lusi;Wang Bei(Department of Epidemiology and Health Statistics,School of Public Health,Southeast University,Nanjing 210009,China;Wuxi Xinwu District Center for Disease Control and Prevention,Wuxi 214028,China;Wuxi Center for Disease Control and Prevention,Wuxi 214000,China;Department of Healthcare Associated Infection Control,Wuxi Second People’s Hospital,Wuxi 214002,China)
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2020年第2期244-248,共5页
Chinese Journal of Epidemiology
基金
中央高校基本科研业务费专项资金(2242016K40025)
江苏省研究生科研与实践创新计划(KYCX17_0184)。
