摘要
目的观察胃香乐方对功能性消化不良(functional dyspepsia,FD)模型大鼠的防治效果并探讨其机理。方法 60只大鼠随机分为模型组,胃香乐方高、中、低剂量组,莫沙必利对照组和空白组。采用复合病因法复制FD模型,分别以相应药物灌胃14 d,计算胃排空率、小肠推进率,测定血浆胃动素(motilin,MTL)水平,电镜下观察胃窦Cajal间质细胞超微结构并进行定量分析。结果莫沙必利组和胃香乐方各组胃排空率、小肠推进率均显著高于模型组(P<0.05),尤以中剂量组和莫沙必利组效果最好;与模型组相比,莫沙必利组和胃香乐方各组MTL均明显升高(P<0.01,P<0.05),尤以中剂量组效果最佳;与模型组比较,莫沙必利组和胃香乐方各组大鼠胃窦Cajal间质细胞超微结构均明显改善;与模型组及莫沙必利组比较,胃香乐方各组线粒体数量和Vv、δ、δm均明显增加(P<0.01,P<0.05)。结论胃香乐方具有促进胃肠动力、防治功能性消化不良的作用。
Objective To explore the preventive and therapeutic effect and mechanisms of Weixiangle prescription(WXP) on functional dyspepsia rats. Methods 60 rats were randomly divided into model group, big-dosage, medium-dosage and small-dosage group of WXP, mosapride group and control group. Each was respectively perfused with corresponding drug for 14 days after the functional dyspepsia models were duplicated by complex etiological method. The gastric emptying rate and the small intestine propulsion rate were calculated, the motilin level of blood plasma was measured, the ultrastructure of gastric antrum Cajal interstitial cells was observed under electric microscope, and quantitative analysis of which were carried out. Results Compared with control group, the gastric emptying rate and the small intestine propulsion rate of mosapride group and each WXP group increased obviously(P<0.05), especially the medium-dosage group and mosapride group. Compared with model group, the MTL level of mosapride group and each WXP group significantly increased(P<0.01 or P<0.05), especially the medium-dosage group. Compared with control group,the gastric antrum Cajal interstitial cells ultrastructure of mosapride group and each WXP group were obviously improved. Compared with control group and mosapride group, the number of mitochondria and Vv, δ, δm of each WXP group increased obviously(P<0.01 or P<0.05). Conclusion WXP has the effect of promoting gastrointestinal motility and treating functional dyspepsia.
作者
禄保平
陈晓乐
刘湘花
LU Baoping;CHEN Xiaole;LIU Xianghua(Basic Medical School,Henan Province of Traditional Chinese Medicine,Henan Province,Zhengzhou 450046,China;Grade 2016 PHD Candidate,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)
出处
《中国中医药现代远程教育》
2020年第1期118-120,共3页
Chinese Medicine Modern Distance Education of China
基金
河南省高等学校重点科学研究项目【No.16A360022】
河南中医药大学河南省研究生教育创新培养基地导师创新基金项目【No.2016CXJDD05】
关键词
胃香乐方
消化不良
胃肠运动
胃动素
胃窦Cajal间质细胞
超微结构
动物实验
大鼠
Weixiangle prescription
functional dyspepsia
gastrointestinal motility
motilin
gastric antrum Cajal interstitial cells
ultrastructure
animal experiment
rat
