摘要
目的探讨参附注射液(SF)对血管性痴呆(VD)大鼠学习记忆功能及海马组织病理学的影响。方法对健康雄性SD大鼠采用双侧颈总动脉反复夹闭再通同时腹腔注射硝普钠降压方法制作VD模型,选出造模成功者随机分为模型组及药物组,另以条件匹配的大鼠为假手术组。各组再随机分为7、15 d、1月时间点亚组。药物组每日腹腔注射SF(10 mL/kg),模型组和假手术组每日注射等量生理盐水。Morris水迷宫检测大鼠空间学习记忆能力,HE染色、尼氏染色和透射电镜检测海马CA1区组织病理学改变。结果模型组大鼠各时间点的逃逸潜伏期(EL)均明显延长(P<0.01);药物组大鼠各时间点的EL较模型组明显缩短(P<0.05),但仍长于假手术组(P<0.01)。光镜下模型组大鼠各时间点的海马锥体细胞减少甚至脱失,残存锥体细胞明显肿胀,出现核固缩等。经SF治疗后上述病理损伤程度减轻。Nissl染色显示模型组大鼠海马组织锥体细胞尼氏体减少或消失。药物组比模型组有所改善,胞浆中尼氏体较丰富。模型1月组大鼠海马组织超微结构完整性破坏,突触前膜内小泡数量减少,SF治疗组突触结构和数量明显改善。结论SF可以改善VD大鼠的学习记忆功能障碍和组织病理学损害,改善痴呆症状。
Objective To observe the effect of Shenfu injection(SF)on learning and memory ability and pathological changes of hippocampus in vascular dementia(VD)rats.Methods The VD models,established by repeatedly clipping the common carotid arteries(CCA)of the rat in combination with an intraperitoneal injection of sodium nitroprusside solution in anesthetized SD rats,were randomly divided into two groups:model group and SF treated group,and another condition-matched rats were selected as the sham-operated group in which the bilateral CCA were separated but with neither CCA occlusion nor intraperitoneal injection of sodium nitroprusside solution.Above 3 groups were further divided into three time point subgroups respectively:7d,15d and 1m subgroups after model-established operation.The SF treated group were intraperitoneally treated with SF(10mL/kg/d),while the model and sham-operated groups were given the same volume of normal saline as SF treated group.At different time point,spatial learning and memory abilities of rats were assessed by Morris water maze task and the microcosmic pathological changes in hippocampal CA1 area were comparatively observed in HE straining,Nissl staining slices through microscopy and transmission electron microscopy.Results The escape latency(EL)of VD rats was significantly higher than sham operation group(P<0.01)While reduced(P<0.05)in SF treated rats.Pathology testing showed that pyramidal cells in CA1 area of hippocampus reduced or even dropped out in VD rats.At the same time,cells were swelling with nucleus pyknosis.Moreover,the number of Nissl bodies reduced or even disappeared,accompanied by glial cells hyperplasia.With treatment of SF,the damages mitigated.Transmission electron microscopy showed damages of integrity and structure in hippocampus of VD rats(1m),accompanied by a decrease in the number of synaptic vesicles while SF relieved the damages.Conclusion SF can ameliorate dementia symptoms and enhance learning and memory in VD rats to some extent.
作者
李乃选
刘红霞
潘虹
施真
于腊梅
LI Naixuan;LIU Hongxia;PAN Hong;SHI Zhen;YU Lamei(Department of Interventional Medicine,Yantai Affiliated Hospital of Binzhou Medical University,Yantai 264003,P.R.China;Department of Physiology,School of Basic Medicine,Binzhou Medical University)
出处
《滨州医学院学报》
2019年第6期401-406,共6页
Journal of Binzhou Medical University
基金
山东省中医药科技发展计划(2015-273)
山东省中医药科技发展计划(2019-0501)
滨州医学院科技计划(BY2011KJ037)
关键词
参附注射液
血管性痴呆
病理学改变
学习记忆
Senfu injection
vascular dementia
pathology changes
learning and memory ability
