摘要
目的探讨抑制Rab11表达对膀胱癌T24细胞增殖和侵袭的影响。方法将Rab11 si RNA转染于人膀胱癌T24细胞系,用免疫印迹法检测干扰效率。通过CCK8、细胞周期检测及基质胶侵袭实验,分析抑制Rab11表达对细胞增殖、细胞周期和侵袭的影响。通过免疫印迹法及实时PCR检测抑制Rab11表达对细胞周期相关因子cyclin D1、cyclin E及侵袭相关因子基质金属蛋白酶9(MMP9)表达量的影响。结果抑制Rab11蛋白表达能够抑制膀胱癌细胞的增殖和侵袭,并下调细胞周期相关因子cyclin D1、cyclin E及侵袭相关因子MMP9的蛋白和m RNA表达量。结论 Rab11可能作为肿瘤蛋白参与膀胱癌的疾病进展。
Objective To investigate the effect of inhibition of Rab11 expression on the proliferation and invasion of human bladder cancer cell line T24. Methods T24 cells were transfected with Rab11 si RNA,and RNA interference efficiency was determined by performing Western blotting. The effect of inhibition of Rab11 expression on cell proliferation,cell cycle progression,and cell invasion was analyzed by performing CCK8,cell cycle detection,and Matrigel invasion assays,respectively. Expression of cell cycle-related proteins cyclin D1 and cyclin E and invasion-related protein matrix metalloproteinase 9（MMP9） was determined by performing Western blotting and RT-PCR. Results Inhibition of Rab11 expression inhibited the proliferation and invasion of bladder cancer cells and downregulated the expression of cell cycle-related proteins cyclin D1 and cyclin E and invasion-related protein MMP9. Conclusion Our results suggest that Rab11 functions as a tumor protein and is involved in the progression of bladder cancer.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2018年第3期247-250,259,共5页
Journal of China Medical University
基金
辽宁省科学技术计划(2015225009)
关键词
Rab11
膀胱尿路上皮癌
增殖
侵袭
Rabl 1
bladder urothelial carcinoma
proliferation
invasion
