摘要
目的探讨血清神经膜蛋白2(NRSN2)在鼻咽癌(NPC)患者血清中的表达,分析其与EB病毒(EBV)抗体及EBV-DNA的关系。方法选取2016年4月至2018年5月期间本院收治的NPC患者120例为研究组,同时期体检中心健康者56例为对照组。采用酶联免疫吸附试验(ELISA)法检测两组血清VCA-IgA、EBNA-IgA、EA-IgG表达,利用荧光定量PCR技术检测两组血清NRSN2和EBV-DNA表达,采用ROC曲线分析血清NRSN2水平对NPC的诊断价值,利用Spearman分析血清NRSN2与鼻咽癌EBV抗体及EBV-DNA的关系,采用非条件logistic多元回归分析NPC发生的影响因素。结果研究组血清VCA-IgA、EBNA-IgA及EA-IgG阳性率均显著高于对照组(P<0.05);研究组血清NRSN2水平明显高于对照组(P<0.05),研究组EBV-DNA阳性表达率明显高于对照组(P<0.05);ROC曲线结果显示,血清NRSN2水平对NPC疾病诊断的AUC为0.759,灵敏度为63.33%,特异度为80.36%;NPC患者血清NRSN2与VCA-IgA、EBNA-IgA、EA-IgG和EBV-DNA均呈正相关(P<0.05);多元回归分析结果显示,VCA-IgA、EBNA-IgA、EA-IgG、EBV-DNA以及NRSN2表达对诊断NPC具有辅助预测价值(P<0.05)。结论NPC患者血清NRSN2水平上调,与患者血清VCAIg A、EBNA-IgA、EA-IgG和EBV-DNA水平正相关,可为NPC的预测、诊断和治疗提供一定参考。
Objective To investigate the expression of serum Neurensin 2( NRSN2) in patients with nasopharyngeal carcinoma( NPC),and to analyze its relationships with epstein-barr virus( EBV) antibody and EBV-DNA. Methods 120 patients with NPC admitted to our hospital from April 2016 to May2018 were selected as the study group,and 56 healthy people in the physical examination center were selected as the control group. Enzyme linked immunosorbent assay( ELISA) was used to detect the expressions of serum viral capsid antigen-IgA( VCA-IgA),nuclear related tumor antigen-IgA( EBNA-IgA) and early antigen-IgG( EA-IgG). The expression of serum NRSN2 and EBV-DNA were detected by fluorescence quantitative polymerase chain reaction( q PCR). Receiver operating characteristic( ROC) curve was used to analyze the diagnostic value of serum NRSN2 level for NPC. Spearman was used to analyze the relationship between serum NRSN2 and EBV antibody with EBV-DNA in nasopharyngeal carcinoma,multivariate logistic regression analysis was used to analyze the risk factors of NPC. Results The positive rates of serum VCAIg A,EBNA-IgA and EA-IgG in the study group were significantly higher than those in control group( P <0. 05);the level of serum NRSN2 and the positive expression rate of EBV-DNA in the study group was significantly higher than that in the control group( P < 0. 05);ROC curves showed that the area under curve( AUC) of serum NRSN2 level in diagnosing NPC disease was 0. 759,with sensitivity 63. 33%,specificity80. 36%;there were significant positive correlations between serum NRSN2 with VCA-IgA,EBNA-IgA,EA-IgG and EBV-DNA in NPC patients( P < 0. 05);multivariate regression analysis showed that VCA-IgA,EBNA-IgA,EA-IgG,EBV-DNA and NRSN2 expressions were supportive predictive value for diagnostic NPC( P < 0. 05). Conclusions Up-regulation of serum NRSN2 levels in NPC patients is positively correlated with serum VCA-IgA,EBNA-IgA,EA-IgG and EBV-DNA levels,which may provide a reference for the prediction,diagnosis and treatment of NPC.
作者
张宏伟
陈菊华
Zhang Hongwei;Chen Juhua(Department of Laboratory,Hanzhong People's Hospital,Hanzhoung 723000,China;Department of Pathology,Hanzhong Central Hospital,Hanzhoung 723000,China)
出处
《中国医师杂志》
CAS
2019年第11期1688-1692,共5页
Journal of Chinese Physician
