摘要
目的探讨旋毛虫成虫排泄 分泌蛋白(Adult worm excretory secretory protein,AES)对卵清蛋白(Ovalbumin,OVA)诱导的小鼠变应性鼻炎的保护作用。方法18只雌性BALB/c小鼠随机分为3组:A组为空白对照组,B组为变应性鼻炎组,C组为AES治疗组。A组小鼠给予PBS;B组小鼠经腹腔注射抗原佐剂混悬液进行基础致敏,隔日1次,共7次,后以5%OVA溶液滴鼻进行局部激发,每日1次,共7次,诱导建立变应性鼻炎模型;C组小鼠在诱导变应性鼻炎基础上,于基础致敏和局部激发时分别加入25μg AES进行干预。末次激发后观察各组小鼠30 min,并进行行为学评分;采用酶联免疫吸附试验检测小鼠血清中IFN γ、IL 4、IL 5、IL 10及TGF β水平变化,HE染色后镜下观察小鼠鼻黏膜病理改变。结果3组小鼠行为学评分差异有统计学意义(F=110.12,P<0.01);B组小鼠行为学评分显著高于A组[(7.17±0.75)分和(1.33±0.52)分,P<0.01],且鼻黏膜病理损伤更为显著;经AES治疗后,C组小鼠行为学评分[(3.83±0.75)分]显著下降(P<0.01),鼻黏膜病理损伤亦显著缓解。3组小鼠血清中IFN γ水平差异有统计学意义(F=7.50,P<0.01),B组小鼠血清中IFN γ水平显著低于A组(P<0.05);经AES治疗后,C组小鼠血清中IFN γ水平较B组显著升高(P<0.05)。3组小鼠血清中IL 4(F=470.81,P<0.01)、IL 5水平(F=68.20,P<0.01)差异均有统计学意义,B组小鼠血清中IL 4、IL 5水平均显著高于A组(P均<0.01);经AES治疗后,C组小鼠血清中IL 4、IL 5水平均较B组显著降低(P均<0.01)。3组小鼠血清中IL 10(F=174.91,P<0.01)、TGF β水平(F=9.39,P<0.01)差异均有统计学意义;经AES治疗后,C组小鼠血清中IL 10、TGF β水平均显著高于B组(P均<0.05)。结论旋毛虫AES对OVA诱导的小鼠变应性鼻炎有显著的保护作用。
Objective To investigate the protective effect of excretory-secretory protein(AES)from adult Trichinella spiralis on ovalbumin(OVA)-induced allergic rhinitis in mice.Methods Eighteen female BALB/c mice were randomly divided into three groups,including the blank control group(Group A),OVA-induced rhinitis group(Group B)and AES treatment group(Group C).Mice in Group A were given PBS.Mice in Group B were intraperitoneally injected with antigen adjuvant suspensionfor systemic sensitization,once every other day for seven times;then,local excitation was intranasally induced with 5%OVA solution once a day for seven times to establish a mouse model of allergic rhinitis.In addition to induction of allergic rhinitis,mice in Group C were given 25μg AES at baseline sensitization and local excitation.Following the final challenge,mice were observed for 30 min in each group,and the behavioral score was evaluated.The serum levels of IFN-γ,IL-4,IL-5,IL-10 and TGF-βwere determined using an enzyme-linked immunosorbent assay in mice,and the pathological changes of mouse nasal mucosa were observed under a microscope.Results There was a significant difference in the mouse behavioral scores among the three groups(F=110.12,P<0.01).The mouse behavioral score was significantly higher in Group B than in Group A(7.17±0.75 vs.1.33±0.52,P<0.01),and more remarkable pathological damages of mouse nasal mucosa were seen in Group B than in Group A,while the mouse behavioral score was significantly decreased in Group C than in Group B(P<0.01),and the pathological damages of mouse nasal mucosa remarkably alleviated in Group C relative to Group B.There was a significant difference in serum IFN-γlevel among the three groups(F=7.50,P<0.01)and the serum IFN-γlevel in Group B was significantly lower than in group A and C(both P<0.05).There were significant differences in serum IL-4(F=470.81,P<0.01)and IL-5 levels(F=68.20,P<0.01)among the three groups,and significantly greater serum IL-4 and IL-5 levels were detected in Group B than in Group A(P<0.01),while significantly lower serum IL-4 and IL-5 levels were detected in Group C than in Group B(P<0.01).There were significant differences in serum IL-10(F=174.91,P<0.01)and TGF-βlevels(F=9.39,P<0.01)among the three groups,and significantly greater serum IL-10 and TGF-βlevels were seen in Group C than in Group B(both P<0.05).Conclu Conclusion T.spiralis AES has a remarkable protective activity against OVA-induced allergic rhinitis in mice.
作者
袁圆
王黎源
梅娟
程洋
王伟
褚亮
唐洁
李楠
李徽徽
贺文欣
杨浪浪
陈杰
白永生
吴玥
梁金宝
孙思颖
张祥
杨小迪
YUAN Yuan;WANG Li-Yuan;MEI Juan;CHENG Yang;WANG Wei;CHU Liang;TANG Jie;LI Nan;LI Hui-Hui;HE Wen-Xin;YANG Lang-Lang;CHEN Jie;BAI Yong-Sheng;WU Yue;LIANG Jin-Bao;SUN Si-Ying;ZHANG Xiang;YANG Xiao-Di(Department of Basic Medical Sciences,Bengbu Medical College,Bengbu 233000,China;Anhui Provincial Key Laboratory of Infection and Immunity,China;The Second Affiliated Hospital of Bengbu Medical College,China;Department of Pathogenic Infection and Immunity,Wuxi Medical School,Jiangnan University,China;The First Affiliated Hospital of Bengbu Medical College,China)
出处
《中国血吸虫病防治杂志》
CAS
CSCD
北大核心
2019年第5期504-509,共6页
Chinese Journal of Schistosomiasis Control
基金
国家自然科学基金（81441120）
安徽高校科研创新平台团队项目（2016-40）
安徽省学术和技术带头人及后备人选科研活动经费资助项目（2018H174)
安徽省高等学校自然科学研究重点项目（KJ2018A0227）
蚌埠医学院自然科学研究项目（BYKY1757）
蚌埠医学院自然科学研究重点项目（BYKY1624ZD）
蚌埠医学院科技发展基金项目（BYKF1853)
关键词
旋毛虫
排泄
分泌蛋白
变应性鼻炎
卵清蛋白
保护作用
小鼠
Trichinella spiralis
Excretory secretory proteins
Allergic rhinitis
Ovalbumin
Protective effect
Mouse
