摘要
探究东北地区栽培甜菜直接再生体系的建立,以期为基因工程手段培育甜菜品种奠定基础。本研究以甜菜子叶节和叶柄为外植体,研究不同消毒方法、不同激素水平对诱导甜菜不定芽和不定根再生的影响。结果表明,最佳消毒方法为:剥除种球外壳获取种胚,并使用75%酒精30 s+0.1%HgCl25 min消毒;诱导不定芽的最佳激素配比为:MS+6-BA 0.5 mg/L+NAA 0.05 mg/L,在此培养条件下,子叶节和叶柄的不定芽诱导率均达到最大值,分别为14.44%、11.67%;诱导不定根的最佳激素配比为:1/2MS+NAA 0.05 mg/L,诱导率达到93.33%。本研究初步建立了东北甜菜直接再生体系,为下一步建立遗传转化体系及甜菜遗传改良奠定基础。
The study aims to establish a direct regeneration system for cultivated sugar beet in northeast China,and lay a foundation for the cultivation of sugar beet varieties by genetic engineering.In this study,cotyledonary sections and petioles were used as explants to study the effects of different disinfection methods and hormone levels on the induction of adventitious bud and adventitious root.The results showed that:the best disinfection method was stripping the seed shell to obtain seeds and the lowest pollution rate was performed using 75% alcohol for 30 s and 0.1% HgCl2 for 5 min;the optimum ratio of hormone to the induction of adventitious bud was MS+6-BA 0.5 mg/L+NAA 0.05 mg/L;the induction rate of adventitious bud using both cotyledon and petiole were biggest with 14.44% and 11.67%,respectively;the optimum ratio of hormone to induce adventitious root was 1/2MS+NAA 0.05 mg/L,and the induction rate was 93.33%.This study preliminarily establishes the direct regeneration system for sugar beet in northeast China,laying the foundation for the establishment of genetic transformation system and genetic improvement of sugar beet.
作者
李洪丽
端木慧子
马春泉
Li Hongli;Duanmu Huizi;Ma Chunquan(Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education/Heilongjiang University,Harbin 150500;Key Laboratory of Molecular Biology,College of Heilongjiang/School of Life Sciences,Heilongjiang University,Harbin 150080)
出处
《中国农学通报》
2019年第30期31-36,共6页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金“甜菜M14品系BvM14-RIN4蛋白盐胁迫响应蛋白互作网络的研究”(31801426)
黑龙江省自然科学基金“基于RNAseq技术筛选甜菜M14品系块根变异性状相关基因”(C2015026)
黑龙江省省属高等学校基本科研业务费基础研究项目“甜菜再生体系的建立及优化”(KJCXZD201718)
黑龙江省省属高等学校基本科研业务费基础研究项目“甜菜M14品系与二倍体栽培甜菜的比较转录组学研究”(KJCXZD201717)
黑龙江省高校创新团队建设计划项目“寒区植物重要基因资源的挖掘与种质创新”(2014TD004)
黑龙江省普通高等学校青年创新人才培养计划“甜菜M14品系BvM14-RIN4基因耐盐功能及其蛋白调控机理研究”(UNPYSCT-2018008)
关键词
甜菜
组织培养
直接再生
子叶节
叶柄
诱导不定芽
诱导不定根
sugar beet
tissue culture
direct regeneration
cotyledonary section
petiole
induced adventitious bud
induced adventitious root
