摘要
目的:探究缺血再灌注损伤大鼠缺血侧运动皮层中M2型小胶质细胞衍生的外泌体表达,观察电针曲池、足三里穴对缺血再灌注损伤大鼠运动功能的影响及其可能机制。方法:36只雄性SD(Sprague-Dawley)大鼠随机分为假手术组、模型组和电针组各12只,采用Zea-Longa神经行为学评分进行神经功能缺损评定;Catwalk跑台实验评估运动功能;免疫组化法标记缺血侧运动皮层M2型小胶质细胞阳性细胞数量,免疫荧光双标法标记其外泌体蛋白CD81的表达。结果:干预7d后,电针组与模型组大鼠相比,Zea-Longa评分显著降低(P<0.05);步行速度增快(P<0.01),持续时间减少(P<0.05),大鼠爪印面积显著性增加(P<0.01);缺血侧运动皮层M2型小胶质细胞及其分泌的外泌体蛋白CD81增多,差异均有统计学意义(P<0.01)。结论:电针曲池、足三里穴可促进缺血侧运动皮层M2型小胶质细胞外泌体的分泌,发挥神经保护作用,有效改善缺血再灌注损伤大鼠的运动功能。
Objective:To investigate the expression of M2 microglia-derived exosomes in the ischemic motor cortex of rats with ischemia-reperfusion injury,and to observe the effects of electroacupuncture at Quchi and Zusanli on motor function in rats with ischemia-reperfusion injury and possible mechanisms.Methods:Thirty-six male Sprague-Dawley rats were randomly divided into sham operation group,model group and electroacupuncture group ( n =14 each).Zea-Longa neurobehavioral score was used for neurological deficit assessment.Catwalk treadmill experiment was used to evaluate motor function.The number of M2 microglia positive cells in the ischemic motor cortex was marked by immunohistochemistry,and the expression of exosome protein CD81 was detected by immunofluorescence double labeling.Results:After 7 days of electroacupuncture intervention,the Zea-Longa score was significantly reduced ( P < 0.05 ),the walking speed increased significantly ( P < 0.01 ),the duration decreased significantly ( P < 0.05 ),the paw print area of the rats increased significantly ( P < 0.001 ),and the number of M2 type microglia and the secretion of exogenous protein CD81 increased significantly in the ischemic motor cortex ( P < 0.01 ) in the electroacupuncture group as compared with those in the model group.Conclusion:Electroacupuncture at Quchi and Zusanli can promote the secretion of M2 type of microglia exosomes in the ischemic motor cortex,play a neuroprotective role and effectively improve the motor function of rats with ischemia-reperfusion injury.
作者
金婷婷
柳维林
李钻芳
李建鸿
张嘉泳
李乐
张宇豪
陈立典
陶静
Yang Minguang
Jin Tingting;Liu Weilin;Yang Minguang(College of Rehabilitation Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)
出处
《中国康复》
2019年第8期395-398,共4页
Chinese Journal of Rehabilitation
基金
国家自然科学基金(81373778)
福建省自然科学基金项目(2016J01397)
关键词
缺血再灌注
M2型小胶质细胞
外泌体
电针
cerebral ischemia
M2 type of microglia
exosomes
electroacupuncture
