摘要
目的开发一种快速定量检测米面等食品中米酵菌酸的方法。方法首先,制备米酵菌酸-牛血清白蛋白偶联物(bongkrekic acid-bovine serum albumin,BA-BSA),作为检测卡的包被原料包被至硝酸纤维素膜上,用本公司自制BA单克隆抗体与时间分辨微球标记放入微孔中,然后将包被后的膜和标记后的微孔组装成快速检测卡。结果快速检测卡的线性范围为1~100 ng/mL,前处理采用碱性甲醇水溶液提取样本中米酵菌酸。结论本方法快速、准确、灵敏度高,适合样品中米酵菌酸的检测。
Objective To develop a method for rapid and quantitative detection of bongkrekic acid in foods that made of rice and wheat. Methods Bongkrekic acid-bovine serum albumin conjugate (BA-BSA) was synthetized and coated on the nitrocellulose membrane at first, and then BA monoclonal antibody produced in Eugene was labeled by TRF microspheres and was freeze-dried in microwells. Membrane and microwell that prepared before were assembled into test kits. Results The BA test kits had a wide detection range (1-100 ng/mL), and the tris-methanol reagent could be easily used for extracting BA from food samples. Conclusion This method is rapid, accurate and sensitive, which is suitable for detecting bongkrekic acid in feed samples.
作者
张小波
温国原
辛苗苗
郑嘉庚
李峰
ZHANG Xiao-Bo;WEN Guo-Yuan;XIN Miao-Miao;ZHENG Jia-Geng;LI Feng(Shanghai Eugene Biotech Co, Ltd., Shanghai 201413, China)
出处
《食品安全质量检测学报》
CAS
2019年第11期3584-3589,共6页
Journal of Food Safety and Quality
关键词
米酵菌酸
时间分辨荧光
快速检测
免疫层析技术
bongkrekic acid
time-resolved fluorescence
rapid test
immunochromatography
