摘要
目的探讨米酵菌酸抑制细胞衰老凋亡作用和可能机制。方法不同生理浓度米酵菌酸在体外作用于人肝癌细胞HepG2细胞株,倒置显微镜下观察细胞形态变化;通过噻唑蓝(MTT)还原反应观察细胞增殖和活性;采用罗丹明/碘化丙啶(Rh123/PI)双染法,流式细胞仪(FCM)分析线粒体膜电位与细胞存活关系;碘化丙啶测定细胞周期,FCM分析细胞生长周期的变化。结果0.1～27.3μmol/L米酵菌酸作用后的HepG2细胞存活率为107.8%～130.0%,空白组为100%,差异有统计学意义(P<0.05);细胞增殖率以及Rh123+PI-、Rh123-PI+、Rh123-PI-及Rh123+PI+百分率与空白对照组比较差异也有统计学意义(P<0.01)。结论米酵菌酸可以抑制HepG2细胞的衰老凋亡,使S+G2/M期细胞增多。
Objective To investigate the effects and possible mechanism of bongkrekic acid （BA） on inhibition of the cell caducity. Methods HepG2 cell was incubated with different concentrations of BA. The morphology change of HepG2 cell was observed under the inverted microscope. The cell proliferation and viability were determined by 3-（4,5- dimethylthiazol-2yl）-2, 5 dipheyltetrazolium bromide （MTT） reduction assay. Rh123/PI dual fluorescent staining was carried out to evaluate cells mitochondrial function and apoptosis by Flow CytoMeter analysis. PI fluorescent staining was used to detect the cell cycle, and the Flow CytoMeter was used to examine the change of cell cycle. Results The livability of HepG2 cell incubated with BA （concentrations： 0.1-27.3 μmol/L） was 107.8%-130.0%, and the control group was 100%（P〈0.05）; the reproducibility and the percentage of Rh123^＋PI^- , Rh123^-PI^＋, Rh123^- PI^- and Rh123^＋PI^＋ compared to normal control group, and there was statistically difference between the two groups （P〈0.01）. Conclusion BA could inhibit cells apoptosis and make S＋G2/M-phase manifold.
出处
《中国病原生物学杂志》
CSCD
2009年第1期4-7,共4页
Journal of Pathogen Biology
