摘要
目的:研究Klotho对绒膜外滋养细胞系HTR8/SVneo侵袭的影响。方法:用免疫组化检测子痫前期及正常孕妇胎盘组织Klotho蛋白表达情况。实验分组为对照组、Klotho重组蛋白组、Klotho siRNA组。Transwell小室法检测侵袭能力,Western Blot检测细胞蛋白表达水平。结果:子痫前期胎盘组织Klotho表达水平明显低于正常胎盘组织。Klotho重组蛋白处理细胞后,HTR8/SVneo细胞侵袭能力显著增强、其表达的基质金属蛋白酶2(MMP-2)及基质金属蛋白酶9(MMP-9)表达上调。Klotho siRNA处理细胞后,HTR8/SVneo细胞侵袭能力显著减弱、其表达的MMP-2及MMP-9表达显著下调。结论:Klotho可能通过介导MMP-2和MMP-9的表达调节滋养细胞侵袭能力,为子痫前期发病机制的研究提供了新线索。
Objective: To investigate the effect of Klotho on invasion of Extrachorionic trophoblastic cell line HTR-8/SVneo cells. Methods: Klotho protein expression in placenta of preeclampsia and normal pregnant women was detected by immunohistochemistry. Experimental grouping: Normal control group, Klotho recombinant protein group, Klotho SiRNA group. The abilities of cell were measured by Transwell assay. The protein expression were detected by Western blot. Results: The expression of Klotho in placenta of preeclampsia was significantly lower than that in normal placenta. Treatment with Klotho recombinant protein improved the HTR-8/SVneo cell invasion abilities, and upregulated the expression of MMP-2 and MMP-9. The effects were re-versed by treatment with Klotho siRNA. Conclusion: Klotho regulate the invasion of trophoblast may via MMP-2 and MMP-9 expression. It provides a new clue for the study of the pathogenesis of preeclampsia.
作者
薛筱蕾
成芳
范翠芳
雷帝
胥保梅
蒋惠玲
XUE Xiao-lei;CHENG Fang;FAN Cui-fang;LEI Di;XU Bao-mei;JIANG Hui-ling(Department of Obstetrics, The Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, China;Department of Obstetrics, Wuhan University People's Hospital, Wuhan, Hubei 430060, China)
出处
《海南医学院学报》
CAS
2019年第10期754-757,共4页
Journal of Hainan Medical University
基金
乌鲁木齐市科学技术计划项目(Y131310002)~~
