摘要
目的探讨5种病原学检测方法在肺结核病检查中的应用价值。方法应用荧光染色法、固体培养法、环介导等温扩增法(loop-mediated isothermal amplification,LAMP)、Xpert MTB/RIF、线性探针法(line probe assay,LPA)对176例肺结核病患者的同一份痰样本进行检测,比较5种方法的阳性率。结果5 种检测方法的阳性率依次为XpertMTB/RIF(77.27%)> LAMP(73.30%)> LPA(70.45%)>固体培养法(58.52%)>荧光染色法(46.02%)。在唾液痰标本中,LAMP(53.06%)、Xpert MTB/RIF(59.18%)、LPA(49.98%)的阳性率均显著高于荧光染色法(20.41%)和固体培养法(30.61%)。在涂阳患者中,LAMP(97.53%)、Xpert MTB/RIF(100.00%)、LPA(100.00%)的阳性率均显著高于固体培养法(87.65%)。在涂阴患者中,LAMP(52.63%)和Xpert MTB/RIF(57.89%)的阳性率均显著高于固体培养法(33.68%),以上差异均有统计学意义(P <0.05)。结论LAMP、Xpert MTB/RIF、LPA 的阳性率高于荧光染色法、固体培养法,对早期发现传染源、控制肺结核病流行传播具有重要意义。
Objective To disscuss the application value of five etiological methods in examination of pulmonary tuberculosis. Methods The same specimens of 176 pulmonary tuberculosis patients were detected by fluorescence staining,solid culture, loop-mediated isothermal amplification ( LAMP),Xpert MTB/RIF and line probe assay( LPA). The positive rates of the five methods were compared. Results The postive detection rates of Xpert MTB/RIF,LAMP,LPA,solid culture and fluorescence staining were 77. 27%,73. 30%,70. 45%,58. 52% and 46. 02%,respectively. The positive rates of LAMP ( 53. 06%), Xpert MTB/RIF ( 59. 18%) and LPA ( 49. 98%) were significantly higher than that of fluorescence staining ( 20. 41%) and solid culture ( 30. 61%) in Saliva sputum specimens. The positive rates of LAMP ( 97. 53%),Xpert MTB/RIF ( 100. 00%) and LPA ( 100. 00%) were significantly higher than that of solid culture ( 87. 65%) in smear postive patients. The positive rates of LAMP ( 52. 63%) and Xpert MTB/RIF ( 57. 89%) were significantly higher than that of solid culture ( 33. 68%) in smear negative patients,and the differences were statistically significant ( P < 0. 05). Conclusion The postive rates of LAMP, Xpert MTB/RIF and LPA were higher than that of fluorescence staining and solid culture. It's important for early detection of infection sources and control of the spread of pulmonary tuberculosis.
作者
杨健
李妍
王蕊
陈美玲
鲜小萍
张天华
YANG Jian;LI Yan;WANG Rui;CHEN Mei-ling;XIAN Xiao-ping;ZHANG Tian-hua(Shaanxi Provincial Institute for TB Control and Prevention,Xi'an,Shaanxi 710048,China)
出处
《中国卫生检验杂志》
CAS
2019年第9期1029-1031,共3页
Chinese Journal of Health Laboratory Technology
基金
陕西省重点研发计划项目(2018SF-207)
关键词
肺结核
结核分枝杆菌
病原学
荧光染色
固体培养
核酸扩增技术
Pulmonary tuberculosis
Mycobacterium tuberculosis
Etiology
Fluorescence staining
Solid culture
Nucleic acid amplification techniques
