摘要
目的:对咪喹莫特诱导建立小鼠银屑病模型进行改良,建立银屑病持续时间更长、特征更典型的银屑病动物模型。方法:将小鼠随机分为正常组(涂抹凡士林)、模型组[涂抹咪喹莫特乳膏60 mg/(2×3 cm^2·d)]和改良组[涂抹咪喹莫特乳膏60 mg/(2×3cm^2·d)+皮下注射重组小鼠白细胞介素12(rmIL-12)和脂多糖(每周1次)],连续21 d,每组10只。从造模第1天起每日观察小鼠造模部位皮肤,对类似银屑病样皮损的红斑、鳞屑、增厚等症状进行银屑病面积与严重性指数(PASI)评分;造模21 d后处死小鼠,取皮损部位进行组织病理学检查,计算脾指数,酶联免疫吸附实验(ELISA)法检测皮肤中IL-17A、IL-12的含量。结果:自给药第3、2天起,模型组和改良组小鼠皮肤分别出现红斑、鳞屑、增厚等类似银屑病样皮损,均在第12天PASI总分达到峰值;改良组从第11天起,小鼠皮肤红斑、鳞屑、厚度较模型组更明显,且连续9 d PASI总评分显著高于模型组(P<0.05);皮肤组织病理学观察到模型组和改良组小鼠均出现Munro微脓肿、棘层肥厚及真皮层炎症细胞浸润;与模型组比较,改良组小鼠脾指数更高(P<0.05);与正常组比较,模型组和改良组小鼠皮肤中IL-17A、IL-12含量均升高,且改良组升高差异有统计学意义(P<0.05)。结论:建立的改良模型银屑病典型特征维持时间更长。
OBJECTIVE:To establish psoriasis animal model with a longer duration and more typical psoriatic characteristics by modifying psoriasis model induced by imiquimod. METHODS:Mice were randomly divided into normal group(treated with vaseline),model group [treated with Imiquimod cream 60 mg/(2×3 cm^2·d)] and modified group [treated with Imiquimod cream 60 mg/(2×3 cm^2·d)+subcutaneous injection of rmIL-12 and LPS once a week],for consecutive 21 d,with 10 mice in each group. The skin of the model site was observed daily from the first day of modeling. Psoriasis area and severity index (PASI) score was conducted for skin lesions such as erythema, scales and thickening. 21 d after modeling, mice were sacrificed, and histopathological examination of the skin lesions was performed. The spleen index was calculated,and the contents of IL-17A and IL-12 in the skin were detected by ELISA. RESULTS:From the third and second day of medication,erythema,scales and thickening were both observed in model group and modified group respectively. The PASI score reached peak on the 12th day. From the 11th day,erythema,scales and thickening of the modified group were more serious than that in model group. PASI scores of modified group was significantly higher than that of model group for 9 consecutive days(P<0.05). Histopathological observation showed that Munro microabscess,acanthosis and dermal inflammatory cell infiltration occurred in both model group and modified group. Compared with model group,spleen indexes of modified group were higher(P<0.05). Compared with normal group,the contents of IL-17A and IL-12 in mice skin of model group and modified group were both increased,and there was statistical significance in modified group (P<0.05). CONCLUSIONS:The estbalished modified model has the longer duration of typical characteristics of psoriasis.
作者
修媛娟
向翠英
邵振俊
XIU Yuanjuan;XIANG Cuiying;SHAO Zhenjun(Dept. of Research&Development,Huapont Pharm Co.,Ltd., Chongqing 401121,China)
出处
《中国药房》
CAS
北大核心
2019年第9期1187-1191,共5页
China Pharmacy
基金
重庆市社会事业与民生保障科技创新专项(No.cstc2016shmszx130055)
