摘要
目的探究MDM2-p53信号通路在结直肠癌发生和发展中的作用机制,以及p53表达与临床病理参数间的关系,进一步分析其对长期预后的影响。方法收集86例患者的结直肠癌组织及癌旁正常组织。分别通过免疫组化、蛋白印迹、实时荧光定量法测定结直肠癌及癌旁正常组织中p53和鼠双微基因2(MDM2)的表达。采用Kaplan-Meier生存曲线对患者进行预后分析。结果在结直肠癌组织中p53和MDM2的蛋白表达与mRNA表达水平均明显高于癌旁组织(均P<0.01),且二者表达呈现正相关(r=0.785)。结直肠癌组织中p53的表达与肿瘤分化程度、TNM分期、淋巴结转移、浸润深度临床病理特征有关(均P<0.05)。p53高表达组平均总生存时间为(53.92±1.56)个月,明显低于p53低表达组的平均总生存时间(69.16±3.72)个月,差异有统计学意义(χ2=14.78,P<0.01)。结论结直肠癌的发病风险及预后与MDM2-p53信号通路密切相关,p53可作为结直肠癌预后和治疗效果的潜在靶点。
Objective To investigate the mechanism of MDM2-p53 signaling pathway in the development of colorectal cancer and correlation between p53 with clinicopathological parameters, so as to further analyze the effect of p53 on prognosis. Methods The colorectal cancer tissues and the adjacent normal tissues from 86 cases of patients with colorectal cancer were collected. The expression of p53 and murine double minute 2(MDM2) in colorectal cancer and adjacent normal tissues were detected by immunohistochemistry, Western Blot and real-time fluorescence quantitative polymerase chain reaction (RT-PCR). The prognosis of the patients was analyzed by the Kaplan-Meier survival curves. Results The protein expression and the mRNA expression of p53 and MDM2 in colorectal cancer tissues were significantly higher than that in the adjacent non-cancerous tissues (all P<0.01). A positive correlation was observed between the expression of p53 and MDM2 (r=0.785). The expression of p53 in colorectal cancer tissues were correlated well with the degree of tumor differentiation, TNM stage, lymph node metastasis and infiltration depth (all P<0.05). Survival analysis demonstrated that the mean overall survival time in p53 high expression group was (53.92±1.56) months which was significantly lower than that in p53 low expression group of (69.16±3.72) months, and the difference was statistically significant (χ2=14.78, P<0.01). Conclusions The risk and prognosis of colorectal cancer are closely related to the MDM2-p53 signaling pathway. p53 can be used as a potential target for the prognosis and treatment of colorectal cancer.
作者
郝敬鹏
东帅
何彬
韩梅
李梦龙
李鹏昊
郑冰
王晖
Hao Jingpeng;Dong Shuai;He Bin;Han Mei;Li Menglong;Li Penghao;Zheng Bing;Wang Hui(Department of Anorectal Surgery, the Second Hospital of Tianjin Medical University, Tianjin 300211, China)
出处
《国际生物医学工程杂志》
CAS
2019年第1期27-32,共6页
International Journal of Biomedical Engineering
关键词
结直肠癌
P53
鼠双微基因2
临床病理特征
信号通路
预后
Colorectal cancer
p53
Murine double minute 2
Clinicopathological features
Signaling pathway
Prognosis
