摘要
背景:近期研究证实,间充质干细胞的直接治疗作用存在局限性,而其分泌功能越来越受到重视。外泌体是细胞内部的胞内体与细胞膜融合释放到细胞外的膜性囊泡,是细胞与细胞之间进行交流的工具,是间充质干细胞分泌的重要组成部分。目的:对间充质干细胞外泌体在组织修复过程中的作用进行总结分析,以期为再生医学的基础研究和临床防治提供新的方向和思路。方法:以"exosomes,extravesicles,MSCs,mesenchymal stem cells,regenerative medicine"为英文检索词,通过计算机检索PubMed数据库与Embase数据库;以"再生医学,组织修复,外泌体,间充质干细胞"为中文关键词,计算机检索CNKI、万方、维普数据库近10年内文献,纳入描述间充质干细胞来源外泌体的特性及修复作用的文章,排除个例报道、回复信、会议摘要及国内外灰色文献、研究质量较低的文章。结果与结论:间充质干细胞在体内的总体含量很低,存活时间很短,往往达不到起效的时间。间充质干细胞分泌的外泌体含有遗传信息,能够远距离长时间运输,具有作用于靶细胞的能力,其在保护和营养神经组织、血管再生的调控、内脏疾病组织修复、伤口愈合和组织修复、骨骼及软骨的再生等方面具有重要作用。
BACKGROUND: Recent studies have confirmed that direct therapy of mesenchymal stem cells has limitations, and their secretory function has been paid more and more attention. Exosomes are membrane vesicles released from the fusion of intracellular bodies and cell membranes inside the cells, which are tools for cell-to-cell communication and an important part of the secretion of mesenchymal stem cells.OBJECTIVE: To summarize and analyze the role of mesenchymal stem cell exosomes in tissue repair, in order to provide new directions and ideas for basic research and clinical prevention and treatment of regenerative medicine.METHODS: Pub Med database and Embase database were retrieved by computer with the English words of "exosomes, extravesicles, MSCs,mesenchymal stem cells, regenerative medicine";CNKI database, WanFang database and VIP database were retrieved by computer with the Chinese key words of "regenerative medicine, tissue repair, exosome, mesenchymal stem cells". Articles describing the characteristics and repair effects of exosomes derived from mesenchymal stem cells in the past 10 years were included, and case reports, reply letters,summaries of conferences, gray literature at home and abroad, and articles with low quality of research were excluded.RESULTS AND CONCLUSION: Mesenchymal stem cells have a very low overall content in the body, and their survival time is very short,which often fails to reach the effective time. The exosomes secreted by mesenchymal stem cells contain genetic information, which can be transported over a long distance and have the ability to act on target cells. It plays an important role in the protection and nutrition of nerve tissue, regulation of vascular regeneration, tissue repair of visceral diseases, wound healing and tissue repair, bone and cartilage regeneration.
作者
高坤
朱文秀
刘伟东
余伟吉
王立新
曹亚飞
Gao Kun;Zhu Wenxiu;Liu Weidong;Yu Weiji;Wang Lixin;Cao Yafei(Department of Orthopedic Surgery, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518000, Guangdong Province, China;Shenzhen Hospital, Beijing University of Chinese Medicine, Shenzhen 518100, Guangdong Province, China)
出处
《中国组织工程研究》
CAS
北大核心
2019年第13期2107-2112,共6页
Chinese Journal of Tissue Engineering Research
基金
深圳市医疗"三名工程"(SZSM201812066)
项目负责人:曹亚飞~~
