摘要
细菌群落在海洋生物地球化学过程中起着至关重要的作用。分析判别地理距离和环境条件等因素对细菌群落的影响,有助于理解环境变化如何驱动微生物多样性和功能的机制。通过细菌16SrDNA高通量测序的方法,对南海北部水层及沉积表层细菌群落多样性及空间分布进行比较分析,并检验环境因素、地理限制及海底深度等因素对微生物群落的影响。结果表明,约95%的细菌16SrDNA序列归属于γ-变形菌纲、厚壁菌门、蓝细菌门、α-变形菌纲等优势类群。沉积样本中的优势细菌类群主要与粒度、氮(N)等环境参数相关,而水体样本的优势细菌类群与取样深度密切相关。相关性检验结果显示,沉积样本的α-多样性指数与C有着显著的相关关系,而水体样本的α-多样性指数和β-多样性矩阵则显示与取样深度的显著相关;此外,地理距离和海底深度对β-多样性的影响并不显著,这可能与取样空间尺度有关。
Bacterial communities play a vital role in marine biogeochemical processes. Revealing the effects of geographical distance and environmental conditions on bacterial communities is important for better understanding how environmental changes can drive microbial diversity and maintain their functions. In this study, the diversity and spatial distribution of bacterial communities in water and surface sediments of northern South China Sea were analyzed through high-throughput sequencing of bacteria 16S rDNA, and the effects of environmental factors, geographical restrictions and seabed depth on microbial communities were examined. The results showed that about 95% of bacterial 16S rDNA sequences belonged to the dominant phyla/classes Gamma-proteobacteria, Firmicutes, Cyanobacteria, Alpha-proteobacteria, etc. The dominant bacterial groups in sedimentary samples were mainly related to the environmental parameters such as grain size and N, while the dominant bacterial groups of water samples were closely related to sampling depth. The results of correlation test showed that α-diversity indexes of sedimentary samples had significant correlations with C, while both α-diversity indexes and β-diversity distance matrix of water samples showed significant correlations with sampling depth. In addition, geographical distance and depth of the seabed were not detected to have a significant effect on β-diversity, which may be due to the insufficient space scale of sampling.
作者
于淑贤
王寅初
李佳霖
庞云龙
秦松
YU Shu-xian;WANG Yin-chu;LI Jia-lin;PANG Yun-long;QIN Song(Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China;University of Chinese Academy of Sciences,Beijing 100049,China)
出处
《海洋科学进展》
CAS
CSCD
北大核心
2019年第1期102-114,共13页
Advances in Marine Science
基金
中国科学院战略试点项目--热带西太平洋海洋系统物质能量交换及其影响(XDA1102040300)
中国科学院与俄乌白等国科技合作补助专项--中国-波兰联合共建黄渤海海岸带硅藻种质库以及该海域硅藻生物多样性研究(Y760041011)
国家重点研发计划专项--中美合作利用微藻固碳减排关键技术及运行模式推广示范(2016YFE0106700)
山东省科技发展计划项目--莱州湾微咸水与滩涂植物资源综合利用示范(2010GSF10208)
关键词
细菌
高通量测序
16SrDNA
环境影响
bacteria
high-throughput sequencing
16S rDNA
environmental effect
