摘要
【目的】研究分析南海深海沉积物可培养细菌多样性及其生物毒性,为南海深海海洋微生物资源的开发应用提供参考依据。【方法】以传统培养方法分离培养南海深海沉积物中的细菌,基于16S r RNA基因序列对培养细菌多样性进行分析,并利用咸水虾生物致死法测试培养细菌发酵液的生物毒性。【结果】从南海深海沉积物样品中共分离获得可培养细菌71株,分属于3个门(变形菌门、放线菌门和厚壁菌门)、9个属(假单胞菌属、盐单胞菌属、赤细菌属、副球菌属、Salinimonas属、短杆菌属、芽孢杆菌属、微小杆菌属和Fictibacillus属)的27个种。有4株分离菌株的发酵液对咸水虾具有毒理活性,其中以NH11h04-12(Bacillus tequilensis)对咸水虾的致死活性最强,半数致死量(LD50)为0.81 mg/m L,其次是11E416-5(B.subtilis subsp.)和E201-6(B.aquimaris),对咸水虾的LD50分别为1.59和4.39 mg/m L。【结论】南海深海沉积物可培养细菌存在丰富的多样性,以芽孢杆菌属为优势菌属,且部分可培养菌株代谢物有较强的生物毒性,具有潜在的生物医药应用价值。
[Objective ]The present experiment was conducted to investigate diversity and antimicrobial activity of cul- tivable bacteria isolated from deep-sea sediments of the South China Sea, in order to provide reference basis for develop- ment and application of microbial resources in the South China Sea. [Method]The cultivable marine bacteria were isolated from the deep-sea sediment of the South China Sea by conventional culture-dependent method. Then their diversity was analyzed based on 16S rRNA gene sequence. And the lethal biotoxicity of isolated bacteria against brine shrimp were detected. [Result]A total of 71 strains were isolated from the deep-sea sediment of the South China Sea, which belonged to 3 phyla（Proteobacter{a, Actinobacteria, Firmicutes）, 27 species in 9 genera（Pseudomonas, Cobetia, Erythrobacter; Paracoccus, SalinimonaS, Brevibacteffum, Bacillus, Exiguobacteffum, Fictibacillus）. Among them, the fermentation fluids of four strains had biotoxicity against brine shrimp, especially NH1 lh04-12（Bacillus tequilensis） with the highest lethal activity and LD50 of 0.81 mg/mL, followed by 11 E416 -5 （ B. subtilis subsp. ） and E201-6 （ B. aquimar/s）, their LD50 were 1.59 and 4.39 mg/mL, respectively. [Conclusion]The deep-sea sediments of the South China Sea show high diversity of epiphytic microbial species, are dominated by Bacillus sp., and the metabolites of some strains have high biologica toxicity, so which have potential applied value in biomedicine industry.
出处
《南方农业学报》
CAS
CSCD
北大核心
2015年第12期2203-2208,共6页
Journal of Southern Agriculture
基金
国家自然科学基金项目(41566004)
广西科学研究与技术开发计划项目(桂科合14123001-7)
广西自然科学基金项目(2015GXNSFBB139001
2015GXNSFBA139195)
