摘要
建立了测定畜禽肉中残留卡马西平的多波长可见吸收光谱法。在pH值3. 59 Tris-HCl介质中,固绿FCF与卡马西平反应生成具有3个明显负吸收峰的绿色离子缔合物,出现褪色现象。最大负吸收峰位于660nm,另2个负吸收峰分别位于558 nm和424 nm,线性范围均为0. 05～3. 8 mg/L,遵从比尔定律,它们的表观摩尔吸光系数(κ)分别为6. 74×104(660 nm)、5. 71×104(558 nm)和5. 13×104(424 nm) L/(mol·cm),定量限为0. 11 mg/kg(660 nm)和0. 10 mg/kg(424 nm和558 nm)。当用双波长叠加(660 nm+558 nm)法测定时,其灵敏度为1. 25×105L/(mol·cm),定量限为0. 053 mg/kg。当用三波长叠加(660 nm+558 nm+424 nm)法测定时,其灵敏度达1. 76×105L/(mol·cm),定量限为0. 036 mg/kg。用灵敏度最高的三波长法测定生鲜畜禽肉中残留卡马西平含量,回收率为98. 4%～102%,相对标准偏差(n=5)为2. 4%～3. 2%。
A new multi-wavelength visible absorption spectroscopy method for determining residual carbamazepine in livestock and poultry meat was established.In Tris-hydrochloric acid medium of pH=3.59,fast green FCF reacted with carbamazepine formed a green ion association complex with obvious three negative absorption peaks,and a faded phenomenon appeared in visible area.The maximum negative absorption peak was located at 660nm,and the other two negative absorption peaks located at 558nm and 424nm,respectively.Their linear range were within 0.05-3.8 mg/L.Within this range,Beer's law was obeyed.Their apparent molar absorptivity(κ) were 6.74×10^4(660nm),5.71×10^4(558nm),and 5.13×10^4(424nm) L/(mol·cm),respectively,with the quantitation limits being 0.11mg/kg(660nm) and 0.10mg/kg(424nm and 558nm),respectively.When the double wavelength(660nm+558nm) superposition negative absorption spectrometry was used to determine the concentration of carbamazepine,its sensitivity attained 1.25×10^5 L/(mol·cm) and its quantitative limit was 0.053mg/kg.When three-wavelength(660nm+558nm+424nm) superposition negative absorption spectrometry was used to determine the concentration of carbamazepine,its sensitivity attained 1.76×10 ^5L/(mol·cm) and its quantitative limit was 0.036mg/kg.When the most sensitive three-wavelength method was applied to determine the content of residual carbamazepine in the livestock and poultry meat with spiked recoveries,the recovery and RSD%(n=5) were found to be in the ranges of 98.4%-102% and 2.4%-3.2%,respectively.
作者
江虹
吴小燕
胡金来
JIANG Hong;WU Xiaoyan;HU Jinlai(College of Chemistry and Chemical Engineering,Collaborative Innovation Center for Green Development in Wuling Mountain Areas,Yangtze Normal University,Chongqing 408100,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2019年第1期219-223,共5页
Food and Fermentation Industries
基金
重庆市教委科技基金资助项目(KJ1401226)
长江师范学院科技基金资助项目(2017CXX158)
关键词
多波长
可见吸收光谱法
固绿FCF
畜禽肉
残留
卡马西平
multi-wavelength
visible absorption spectroscopy
fast green FCF
livestock and poultry meat
residual
carbamazepine
