摘要
目的探讨微粒皮种植在治疗糖尿病溃疡过程中,溃疡肉芽组织基质金属蛋白酶(MMP)-2、MMP-9的动态变化。方法对18例糖尿病溃疡患者进行微粒皮种植治疗,在治疗前和治疗后7 d、14 d分别采集创面肉芽组织。用酶联免疫法检测肉芽组织中MMP-2、MMP-9浓度,同时观察溃疡愈合情况。结果微粒皮种植治疗后7 d、14 d的溃疡面积在各观察时间点均较治疗前缩小(P<0.05);该组肉芽组织MMP-2浓度逐渐下降,在治疗后第14天时降至最低(P<0.05); MMP-9从治疗后逐渐降低,但各时点与治疗前相比,差异均无统计学意义;肉芽组织中MMP-2的浓度与溃疡面积大小呈正相关关系(r=0.385,P<0.05)。结论微粒皮种植治疗可降低糖尿病溃疡肉芽组织中MMP的浓度,MMP-2的浓度可能是影响糖尿病皮肤溃疡愈合的相关因素。
Objective To investigate the effect of microskin grafting on matrix metalloproteinase-2(MMP-2)and MMP-9levels in diabetic ulcer.Methods Eighteen cases of diabetic ulcers were treated with microskin grafting.The wound granulation tissues were sampled for detection of MMP-2and MMP-9levels before and7d,14d after treatment using Enzyme-Linked Immunosorbent Assay.The areas of the ulcers were observed and measured.Results The area of ulceration after microskin grafting treatment was significantly reduced at each observation time point compared with that before treatment(P<0.05).The concentrations of MMP-2in the granulation tissues gradually decreased and reduced to the lowest level on the14th day after treatment(P<0.05).MMP-9decreased gradually after treatment,but in each time point there was no significant difference in MMP-9level compared with that before treatment.There was a positive correlation between the concentration of MMP-2in granulation tissue and the size of ulcer(r=0.385,P<0.05).Conclusion Microskin grafting therapy can reduce the concentration of MMP-2in the granulation tissues of diabetic ulcers,and the concentration of MMP-2may be a relevant factor influencing the healing of diabetic ulcers.
作者
戚世玲
朱定衡
底大可
吉苏云
敖贤
黄桃源
何仁亮
QI Shi-ling;ZHU Ding-heng;DI Da-ke(Department of Dermatology,the Fifth Affiliated Hospital of Guangzhou Medical University,Guangzhou 510700,China)
出处
《中国临床新医学》
2018年第12期1206-1209,共4页
CHINESE JOURNAL OF NEW CLINICAL MEDICINE
基金
广东省医学科学技术研究基金项目(编号:A2016602)
关键词
微粒皮
糖尿病
溃疡
基质金属蛋白酶
Microskin
Diabetes mellitus
Ulcer
Matrix metalloproteinase(MMP)
