摘要
为明确各柑橘砧木对根结线虫的抗性水平,为生产上柑橘线虫防控提供有效措施,采用病土接种方法对供试14份柑橘砧木材料进行根结线虫抗性鉴定,并采用人工接种2龄幼虫的方法测定分析防御酶POD(过氧化物酶)、PAL(苯丙氨酸解氨酶)、PPO(多酚氧化酶)的活性变化,探讨抗性材料的抗性机制。抗性测定结果显示:接种后土苦柚根结指数为36.37,根内平均雌虫数为每克根7头,平均卵囊数每克根1.3个,对根结线虫有抗性;酸橘次之,其余砧木均感病。防御酶活性测定结果显示:接种根结2龄幼虫后酶的活性在抗、感材料间差异明显,接种后5～35d,抗性材料土苦柚根部的POD、PAL、PPO活性均高于感病材料,表明抗病材料防御酶反应快,积累量大,能及时阻止线虫的侵染,但酶积累及变化有差异。试验结果说明土苦柚对柑橘根结线虫具有较强的抗性,是比较好的抗性砧木。
To understand the resistance of citrus rootstock to root-knot nematode,and provide an effective control of root knot nematodes,14 citrus rootstocks were selected to investigate their resistance to root-knot nematodes by inoculation of infected soil.Changes in activity of defensive enzymes POD(peroxidase),PAL(phenylalanine aminolase)and PPO(polyphenol oxidase)were determined by artificial inoculation of larvae.The results showed that most rootstocks were susceptible,but“Tukuyou”was highly resistant,with a gall index of 36.37,and an average female and egg mass of 7 and 1.3 per gram in root,respectively.The activities of the enzymes were significantly different between the resistant and susceptible rootstocks,with variation of the resistant rootstock significantly higher than those of the susceptible rootstocks after inoculation.It indicated that defensive enzyme reaction and accumulation of the resistant rootstocks was fast,which could prevent the infection of root-knot nematode in time,but the enzyme accumulation amount and changes were different.“Tukuyou”had strong resistance to citrus root nematodes and was regarded as a good choice of resistance rootstock.
作者
黄金玲
陆秀红
张禹
李秋捷
刘志明
黎起秦
HUANG Jinling;LU Xiuhong;ZHANG Yu;LI Qiujie;LIU Zhiming;LI Qiqin(Institute of Plant Protection,Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests,Nanning 530007,China;College of Agricuture,Guangxi University,Nanning 530005,China)
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2018年第6期25-29,共5页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(31760506)
广西自然科学基金项目(2014GXNSFBA118133)
广西农业科学院科技发展基金项目(桂农科2015YM17
2015JZ42
2016JZ06
2015JZ54)
广西作物病虫害生物学重点实验室基金项目(2016-ST-3)
关键词
柑橘砧木
根结线虫
防御酶
抗性鉴定
citrus rootstock
root-knot nematode
defensive enzyme
resistance evaluation
