摘要
目的筛选逆转卵巢癌细胞耐药性效果最佳的大豆皂苷,并研究其可能的作用机制。方法用4种常见大豆皂苷分别处理耐紫杉醇卵巢癌细胞A2780/PTX,通过检测细胞IC_(50)值,比较其耐药性变化,从中筛选出逆转耐药性效果最佳的大豆皂苷。应用蛋白免疫印迹和激光共聚焦法检测大豆皂苷处理后,A2780/PTX细胞中上皮-间充质转化(epithelial-mesenchymal transition,EMT)相关标志性蛋白的表达情况;Transwell和划痕实验观察细胞迁移能力的变化。结果细胞活力检测结果显示,4种常见的大豆皂苷中,Ac逆转A2780/PTX细胞耐药性的效果最明显;且经大豆皂苷Ac处理48 h后,A2780/PTX细胞中的N-cadherin表达降低,Ecadherin表达升高,细胞的迁移能力降低。结论大豆皂苷Ac能有效降低A2780/PTX细胞的EMT进程,减弱细胞的迁移能力,逆转其耐药性。
To screen the most effective soyasaponins for reversing the drug resistance of ovarian cancer cells,and investigate the possible mechanism.Methods Cell viability assay was used to analyze the drug resistance of A2780/PTX cells treated with four different common soyasaponins respectively,in order to screen out the most effective soyasaponin.Then,the most effective soyasaponin was used to detect the expression of epithelial-mesenchymal transition(EMT)-related marker proteins,including N-cadherin and E-cadherin,with Western blot and confocal microscopy.Finally,transwell assay and wound healing assay were applied to observe effect of soyasaponin on regulating cancer cell migration.Results Compared with other soyasaponins,soyasaponin Ac most effectively reversed the drug resistance of A2780/PTX cells.The expression of N-cadherin decreased while that of E-cadherin increased in A2780/PTX cells when treated with soyasaponin Ac for 48 h.The results of transwell and wound healing assay suggested that soyasaponin Ac also reduced the migration of A2780/PTX cells.Conclusion Soyasaponin Ac can decrease the drug resistance via EMT pathway and weaken the migration ability of ovarian cancer cells.
作者
陆春晓
崔越
何冬旭
马鑫
LU Chun-xiao;CUI Yue;HE Dong-xu;MA Xin(School of Pharmaceutical Sciences and Wuxi Medical School,Wuxi,Jiangsu 214122,China;National Engineering Lab for Cereal Fermentation Technology,Jiangnan University,Wuxi,Jiangsu 214122,China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2018年第9期1249-1253,共5页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 81572940)
