摘要
采用相对和绝对定量同位素标记实验的方法对螺旋藻全蛋白进行定性分析,并选用合理的统筹归类方法对其进行实用性归类。结果表明,共定性检测到螺旋藻蛋白质数为2 085个,其中已知蛋白1 562个;通过序列分类法将869个蛋白共分成233个蛋白超家族与87个(均为已知蛋白)蛋白家族;根据蛋白的性质、结构和功能可分成7大类,发现蛋白超家族在抗氧化、抗癌、抗肿瘤、免疫调节等方面具有重要功能,可被广泛应用于食品、保健和医药等领域。由此可见,螺旋藻活性蛋白具有极大的开发前景。
This research adopted the isobaric tags for relative and absolute quantitation(iTRAQ)method for qualitative analysis of the whole proteins of Spirulina and employed a reasonable overall categorization method to categorize them according to practicability.The results demonstrated that a total of 2 085 Spirulina proteins were qualitatively identified including 1 562 known proteins.Totally,869 annotated proteins could be classified into 233 protein superfamilies and 87 protein(all members are known proteins)families by sequence categorization.Based on their nature,structures and functions,these annotated proteins could be classified into 7 categories.It was found that the protein superfamilies had vital functions such as antioxidant,anticancer,antitumor and immunoregulation,and could be widely used in food,medicinal,chemical industry and other fields.Thus,active proteins of Spirulina have has promising prospects.
作者
王娜
向清豪
赵肖荣
李博生
聂中良
WANG Na;XIANG Qinghao;ZHAO Xiaorong;LI Bosheng;NIE Zhongliang(College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China;Beijing Key Laboratory of Forestry Food Processing and Safety,Beijing Forestry University,Beijing 100083,China;Institute of Spirulina,Beijing Forestry University,Beijing 100083,China;College of Life Sciences,Southwest Forestry University,Kunming 650224,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2018年第16期201-207,共7页
Food Science
基金
公益性行业(林业)科研专项(201304605)
