摘要
目的:探索急性缺血性脑卒中患者血清中可能具有诊断意义的潜在生物标记物。方法:采集急性缺血性脑卒中患者血清73例及健康对照人群血清53例,从血清样本中提取蛋白后,应用相对和绝对定量同位素标记(i TRAQ)定量分析蛋白组学方法检测2组间的差异表达蛋白,采用生物信息学进行鉴定分析,并用western blot的方法进一步验证。绘制受试者工作特征曲线(ROC),计算曲线下面积(AUC),分析差异表达蛋白水平与急性缺血性脑卒中发病情况之间的关联。结果:急性缺血性脑卒中患者血清中前血小板碱性蛋白(PPBP)表达量较健康对照者升高1.47倍(P<0.01);应用western blot验证发现PPBP在急性缺血性脑卒中患者血清中的表达量是健康人群的1.62倍(P<0.01)。以通过PPBP表达含量为检验变量,患病与否为状态变量绘制ROC曲线,AUC=0.7539,95%CI=0.638～0.870(P<0.01)。结论:急性缺血性脑卒中患者血清中PPBP的表达量显著升高,可能是诊断急性缺血性脑卒中潜在的生物标志物。
Abstract Objective： To investigate the serum biomarkers in acute ischemic stroke （AIS） patients that may be of potential diagnostic significance. Methods： Blood serum from 73 acute ischemic strokepatients and 53 health controls was collected. Proteins were isolated from serum samples then analyzed using the proteomics approach of isobaric tagging for relative and absolute quantitation （iTRAQ） to compare the differentially expressed proteins between the two groups. Bioinformatics was used for conformation and analysis, and the western blot was employed for urther validation. The receiver operating characteristic （ROC） curve was created and the area under the curve （AUC） was calculated, and the relationship between differential protein expression and the occurrence of acute ischemic stroke was assessed. Results： Expression of pro-platelet basic protein （PPBP） showed a 1.47-fold increase in the AIS group （P〈0.01） compared to that in the control group. Western blot revealed that PPBP expression in the AIS group was 1.62 times higher than that in the control group （P〈0.01）. With PPBP expression quantity as the test variable and patient or control as the status variable, we created the ROC curve which yielded AUC=0.7539, 95% CI=0.638~0.870 （P〈0.01）. Conclusion： The serum level of PPBP in AIS patients was significantly increased. PPBP may be a potential biomarker for the early diagnosis of AIS.
作者
周罗绮
赵新玲
郭虹
潘邓记
ZHOU Luo-qia;ZHAO Xin-linga;GUO Hongb;PAN Deng-jia(Department of Neurology;Department of Otolaryngology-Head and Neck Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science andTechnology,Wuhan 430030,China)
出处
《神经损伤与功能重建》
2018年第8期379-382,共4页
Neural Injury and Functional Reconstruction
基金
国家自然科学基金(No.81571206)
