摘要
背景:迄今为止尚无一种材料能够完全满足临床使用的所有要求,但是复合材料拥有生物可降解性、生物相容性、骨传导性等方面的优点,成为了目前人工骨修复材料的研究热点。目的:制备碱性成纤维细胞生长因子(basic fibroblast growth factor,b FGF)-聚乳酸羟基乙酸(lactic-co-glycolic acid,PLGA)微球/羟基磷灰石(hydroxyapatite,HAP)/聚左旋乳酸(poly L-lactic acid,PLLA)复合有孔材料,观察其理化特性和生物相容性。方法:用复乳法制备b FGF-PLGA微球,将微球与HAP、有孔PLLA混合,制成6种有孔材料:PLLA,HAP/PLLA,PLGA微球/PLLA,PLGA微球/HAP/PLLA,b FGF/HAP/PLLA,b FGF-PLGA微球/HAP/PLLA;通过粒径分析仪、透射电子显微镜、X射线衍射仪、傅里叶变换红外光谱仪、微机差热天平、扫描电子显微镜等进行相关表征;最后通过骨髓间充质干细胞的细胞毒性实验及骨髓间充质干细胞的增殖情况,分析有孔骨架材料的生物相容性。结果与结论:(1)所制得的b FGF-PLGA微球的粒径约为250 nm,载药率为(26.03±0.17)%,包封率为(90.65±2.68)%,形貌为球形且分散性良好;(2)所制得的复合有孔材料空隙均匀,孔径大小相似,微球及颗粒在复合有孔材料中分布合理;(3)b FGF-PLGA微球/HAP/PLLA,b FGF/HAP/PLLA,HAP/PLLA材料的细胞毒性级别为1级(细胞相对存活率≥80%),说明没有明显毒性或者有轻微毒性;(4)上述6种材料均能促进骨髓间充质干细胞增殖,其中b FGF-PLGA微球/HAP/PLLA复合有孔材料对骨髓间充质干细胞增殖最有利,具有良好的生物相容性。
BACKGROUND:To date,no single material can completely meet the clinical requirements.However,the composite materials characterized by good biodegradability,biocompatibility and osteoconductivity have become a highlight of the artificial bone materials.OBJECTIVE:To synthesize the basic fibroblast growth factor(bFGF)-poly(lactic-co-glycolic acid)(PLGA)microspheres/hydroxyapatite(HA)/poly(l-lactic acid)(PLLA)porous bone scaffolds,and to observe the physicochemical properties and biocompatibility of the composite material.METHODS:The bFGF-PLGA microspheres were prepared by double emulsion method,and then six kinds of materials were made including PLLA,PLLA/HA,PLLA/PLGA,PLLA/HA/PLGA,PLLA/HA/bFGF,and bFGF-PLGA microspheres/PLLA/HA.The characterization of the materials were observed by particle size analyzer,transmission electron microscopy,X-ray diffraction,Fourier transform infrared spectrometer,microcomputer differential thermal balance,and scanning electron microscope.Toxicity of these materials and proliferation of bone marrow mesechymal stem cells seeded onto these materials were analyzed and compared.RESULTS AND CONCLUSION:The average particle size of bFGF-PLGA microspheres was about 250 nm,the average drug-loading capacity was(26.03±0.17)%,and the entrapment percentage was(90.65±2.68)%.The prepared bFGF-PLGA microspheres were spherical and had good dispersibility.In addition,all the six kinds of materials had a porous structure with similar pore diameter,in which the microspheres and particles exhibited a rational distribution.The toxic level of bFGF-PLGA microspheres/PLLA/HA,bFGF/HA/PLLA and HAP/PLLA was graded as 1(with a relative survival rate≥80%),indicating no obvious toxicity or slight toxicity.All these six kinds of composite materials can promote the proliferation of bone marrow mesenchymal stem cells,and the bFGF-PLGA microspheres/PLLA/HA shows the best effects on cell proliferation and has good biocompatibility.
作者
李志跃
朱智波
赵群
向思宇
赵鹏
徐哲伟
Li Zhi-yue;Zhu Zhi-bo;Zhao Qun;Xiang Si-yu;Zhao Peng;Xu Zhe-wei(Department of Orthopedics,Xiangya Third Hospital of Central South University,Changsha 410013,Hunan Province,China;the Central Hospital of Yongzhou,Yongzhou 425006,Hunan Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2018年第6期914-920,共7页
Chinese Journal of Tissue Engineering Research
