摘要
目的:通过急性毒性实验和蓄积毒性实验对藤黄醇提取物进行安全性评价。方法:急性毒性实验采用灌胃1次的给药方式,观察给药期间小鼠的一般情况并采用SPSS软件计算其半数致死量;蓄积毒性实验采用蓄积系数法中递增剂量法进行实验,观察给药期间小鼠的一般情况、检测脏器系数和血液生化指标并对重要脏器进行病理切片。结果:藤黄醇提取物的半数致死量LD50为1.691 g/kg(95%CI:1.149~5.030 g/kg)。藤黄醇提取物的蓄积系数为2.92,有明显蓄积毒性,对肝、肺、脑、脾、肾均有不同程度的影响,第15天累积灌胃藤黄醇提取物剂量达到4.94 g/kg时,蓄积毒性实验中实验组昆明小鼠死亡过半。结论:藤黄醇提取物的急性毒性级别为低毒性,其蓄积毒性表现主要是肝毒性。
Objective： To evaluate the safety of gamboge by means of acute toxicity test, maximal tolerance dose test and cumulative toxicity test. Methods ： The acute toxicity test of ethanolic extract of gamboge was administered via intragastric way at one time. The physiologic circumstance of mice was observed during the test. The median lethal dose was calculated by SPSS sofeware. The cumulative toxicity test was determined by increasing dosage method. The physiologic circumstance of mice was observed. The measurement of organ coefficient and blood analysis were detected and the organs were detected by histopathological study. Results： The median lethal dose of ethanolic extract of gamboges administered by intragastric way was determined to be 1. 691 g/kg in mice from the acute toxicity test. The 95% confidence limit was found to fall in the range of 1. 149 - 5. 030 g/kg. The cumulative coefficient was 2.92. The results of cumulative toxicity test indicated ethanolic extract of gamboges had different effects on the liver,lung, brain, spleen and kidney. Half of mice in the treatment group were died when the cumulative dosage was reached 4.94 g/kg. Conclusion ： The acute toxicity level of ethanolic extract of gamboges was low. The target organ of cumulative toxicity was hver.
出处
《中华中医药学刊》
CAS
北大核心
2017年第10期2546-2550,I0013,共6页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(81473565)
江苏省中医消化病临床医学研究中心项目(BL2014100)
关键词
藤黄
醇提取物
半数致死量
蓄积系数法
gamboge
ethanolic extract
median lethal dose
cumulative coefficient method
