摘要
构建了一种基于AuNP-AuNP-UCNP三联体结构检测双靶标双酚A和雌二醇的传感体系。在三联体结构的制备中,首先合成金纳米颗粒(AuNPs)和上转换纳米颗粒(NaYF_4:Yb,Er,Gd,UCNPs),再对两种纳米颗粒进行表面修饰,分别连接各自的核酸适配体形成两种光学荧光探针,设计一段与两种适配体互补配对的核酸序列,以此互补序列为连接桥,依靠碱基互补配对原则实现三联体结构的制备。用透射电子显微镜(TEM)表征其连接效果。当检测体系同时含有双酚A和雌二醇时,可通过荧光和紫外分光光度计实现高效便捷的双靶标检测。结果表明,反应温度为30℃,pH=7.8时,本方法检测双酚A和雌二醇的线性范围分别为2~200 ng/mL和10~150 ng/mL,检出限分别为0.2和0.5 ng/mL。本方法对双酚A和雌二醇均有较好的特异性识别能力,且三联体结构检测体系的稳定性良好。实际水样中加标回收率均在86.1%~107.4%之间,相对标准偏差<6.8%。本方法在实际水样中环境雌激素的快速高效检测方面有良好的应用前景。
A sensing system based on AuNP-AuNP-UCNP triple structure for efficient detection of dual targets was constructed.In the preparation of triple structure,the gold nanoparticles(AuNPs)and upconversion nanoparticles(NaYF 4:Yb,Er,Gd,UCNPs)were synthesized and surface modified.Then the two nanoparticles and their aptamers were connected to form two kinds of optical fluorescent probes.A nucleic acid sequence that matches with two aptamers was designed,rendering the probes to get close based on the principle of complementary base pairing.On the basis of this,a sensing system with a triple structure was prepared,and its connecting effect was characterized by TEM.With this system,dual targets of bisphenol A and estradiol were efficiently and conveniently detected through quantitative determination by fluorescence and UV spectrophotometer.At reaction temperature of 30℃and pH=7.8,this method exhibited good linear range for determination of bisphenol A and estradiol from 2 ng/mL to 200 ng/mL and from 10 ng/mL to 150 ng/mL,with limits of detection of 0.2 ng/mL and 0.5 ng/mL,respectively.This sensing system with the triple structure owned better specificity to structural and functional analogues,and showed good repeatability and stability.What's more,this sensing system was applied in actual water detection,with the recoveries between 86.1%and 107.4%,and the relative standard deviation below 6.8%.This method showed promising applications in other environmental estrogens in water samples.
作者
李巧凤
任舒悦
王瑜
白家磊
彭媛
宁保安
吕全军
高志贤
LI Qiao-Feng;REN Shu-Yue;WANG Yu;BAI Jia-Lei;PENG Yuan;Ning Bao-An;LYU Quan-Jun;GAO Zhi-Xian(Department of Nutrition and Food Hygiene,College of Public Health,Zhengzhou University,Zhengzhou 540001,China;Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental&Operational Medicine,Tianjin 300050,China)
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2018年第4期486-492,共7页
Chinese Journal of Analytical Chemistry
基金
本文系国家自然科学基金项目(Nos.21477162,81773482)和天津市科技支撑计划项目(No.14ZCZDSF0021)资助
关键词
金纳米颗粒
上转换纳米颗粒
三联体
双酚A
雌二醇
Gold nanoparticles
Upconversion nanoparticle
Triple structure
Bisphenol A
Estradiol
