摘要
目的探讨血浆EBV DNA浓度检测在初诊鼻咽癌患者诊断分型中的应用价值。方法按肿瘤淋巴结转移(Tumor Node Metastasis,TNM)进行临床分期,对57例初诊鼻咽癌患者用荧光定量PCR技术检测血浆内EBVDNA浓度。结果EBV DNA阳性率与患者的性别及年龄段均无相关性;TNM分期中Ⅲ期和Ⅳ期的EBV DNA浓度检测结果的差异有统计学意义(P<0.05),且Ⅳ期较高;随T分期的发展,各期EBV DNA阳性检出率增高,T_2和T_4期EBV DNA浓度检测结果的差异有统计学意义(P<0.05),且T_4期较高;M和N分期患者的差异无统计学意义。结论血浆EBV DNA浓度水平变化与鼻咽癌患者的临床分期呈正相关,可以作为一种初诊鼻咽癌患者的辅助诊断手段。
Objective To investigate the correlation between plasma EBV-DNA level and clinical stage of untreated nasopharyngeal carcinoma(UNPC).Me t h o d s Clinical stage of the UNPC patients was determined by tumor node metastasis(TNM),and the content of EBV DNA in57UNPC patients was detected by fluorescence quantitative PCR.Result EBV-DNA positive rate was not correlated with gender and age of patients,but plasma EBVDNA concentration of stageⅢpatients was higher than stageⅣpatients(P<0.05).EBV-DNA positive rate of patients was increased with the development of T stage progress.The EBV-DNA concentration of stage T4patients was higher than stage T2patients(P<0.05).No significant difference of plasma EBV-DNA concentration between the stage M and stage N patients.Conclusion The level of plasma EBV-DNA concentration is positively correlated with clinical stages of nasopharyngeal carcinoma.Detection of plasma EBV-DNA by fluorescence quantitative PCR is a useful auxiliary method for UNPC diagnosis.
作者
凡任芝
FAN Ren-zhi(Laboratory Center of Provincial Hospital of Anhui Medical University ,Hefei , 230001)
出处
《临床输血与检验》
CAS
2017年第5期462-465,共4页
Journal of Clinical Transfusion and Laboratory Medicine
关键词
实时荧光定量PCR
鼻咽癌
EB病毒
Real-time PCR
Nasopharyngeal carcinoma
Epstein-barr virus EBV
