摘要
目的通过研究miR-30d在人稽留流产胎盘绒毛组织中的表达模式,探讨miR-30d与稽留流产的关系。方法收集2013年1月至2014年12月于石家庄第六医院治疗的稽留流产患者及同期该院1∶1匹配的正常人工流产孕妇为研究对象,分别为稽留流产组(80例)和对照组(80例);将稽留流产组按照年龄不同分为4个亚组:年龄≤25岁组(Y≤25)、26～30岁组(Y 26～30)、31～35岁组(Y 31～35)、≥36岁组(Y≥36)。收集各组绒毛组织,利用qRT-PCR技术和原位杂交技术检测miR-30d在胎盘绒毛组织中的表达及定位。结果两组比较发现miR-30d在稽留流产组绒毛组织中的表达有上调趋势,但尚无显著性差异(P>0.05)。与对照组比较,miR-30d在Y≤25组患者胎盘绒毛组织中表达显著下调(P<0.01),在Y26～30组患者胎盘绒毛组织中表达极显著降低(P<0.01),在Y 31～35组患者胎盘绒毛组织中表达上调(P<0.05),在Y≥36组患者胎盘绒毛组织中表达没有显著变化(P>0.05)。进一步分析发现在Y≤25组中,70%稽留流产患者胎盘绒毛组织中miR-30d表达显著下调(P<0.01);在Y 26～30组,100%稽留流产患者胎盘绒毛组织中miR-30d表达显著下调(P<0.01);而在Y 31～35组和Y≥36组,miR-30d的表达没有明显规律。原位杂交结果显示miR-30d表达主要定位于胎盘绒毛合体滋养层细胞。结论对于小于30岁的稽留流产患者,miR-30d在胎盘合体滋养层中表达下调,可能是引起稽留流产的原因之一。
Objective ： To investigate the relationship between miR-30d and missed abortion by analyzing expression patterns of miR-30d in placenta tissue of human missed abortion.Methods ： The patients with missed abortion treated in the Sixth Hospital of Shijiazhuang from January 2013 to December 2014 were as missed abortion group （ n =80） and the normal abortion pregnant women with 1∶1 matching in the same period as the control group （ n =80）.The missed abortion group was subdivided into four subgroups according to age： ≤25 years group,26-30 years group,31-35 years group,and ≥36 years group.The expression pattern of miR-30d in placenta tissue was analyzed by qRT-PCR,and the location of miR-30d was detected by using in-situ hybridization.Results ： The expression of miR-30d in the villus tissues of the missed abortion group was up-regulated,but there was no significant difference （ P 〉0.05）.Compared with the control group,the expression of miR-30d was significantly down-regulated in placental tissues of patients with ≤25 years old （ P 〈0.01）;the expression in patients with 26-30 years group was significantly decreased （ P 〈0.01）;the expression was up-regulated in the patients with 31-35 years group （ P 〈0.05）;and there was no significant change in the patients with ≥36 years group （ P 〉0.05）.Further analysis showed that the expression of miR-30d was significantly down-regulated in 70% of missed abortion patients with ≤25 years group and 100% of 26-30 years group（ P 〈0.01）,and the expression of miR-30d had no obvious pattern in the 31-35 years group and ≥36 years group .The results of in situ hybridization showed that the positive signals of miR-30d were mainly located in syncytrophoblasts.Conclusions ： These results suggest that the reduction of miR-30d expression may be closely related to the occurrence of missed abortion.
作者
宁安凤
董艺超
朱旗
毛丽梅
马旭
夏红飞
NING An-feng;DONG Yi-chao;ZHU Qi;MAO Li-mei;MA Xu;XIA Hong-fei(Reproductive and Genetic Center of National Research Institute for Family Planning,Beijing 100081;Jiamusi University,Jiamusi 154007;Graduate School,Peking Union Medical College,Beijing 100730;The Sixth Hospital of Shijiazhuang,Shijiazhuang 050051)
出处
《生殖医学杂志》
CAS
2018年第12期1232-1237,共6页
Journal of Reproductive Medicine
基金
国家自然科学基金(81771590)
北京市自然科学基金(7172163)
