摘要
目的：研究远志花粉活力和柱头可授性，以期为远志的有性繁殖和杂交育种工作研究提供科学依据。方法：用I，-KI法，2，3，5-三苯基氯化四氮唑（TTC）法测定不同开花天数（开花前一天、第1天、第2天、第3天、第4天、第5天、第6天、第7天、第8天）的远志花粉活力变化，并比较2种染色方法对远志花粉活力的染色效果。用I，．KI法分5J-JN定1d中不同时间段的远志花粉活力、不同贮藏方法（湿法保存的花朵和干法保存的花朵）的远志花粉活力及不同贮藏条件（4，26，-20，-80℃）条件下贮藏不同时间（2，4，8，24，48，72h）远志花粉活力。用联苯胺-过氧化氢法测定其柱头可授性。结果：①远志的花粉活力随着散粉时间的延长而逐渐降低，在开花后第4天花粉活力最高；②在一天8：00-18：00中，10：OO-16：00的花粉活力较强；③干燥花粉在一20。C贮藏的条件下能延长花粉的贮藏时间；④开花后第5天柱头可授性最强。结论：可以采集花后第4天的花粉对花后第5天的柱头进行人工授粉杂交，为远志良种选育提供理论依据。
Objective： To study the pollen viability and stigma receptivity of Polygala tenuifolia, so as to provide a scientific basis for sexual reproduction and breeding of new varieties. Method： The pollen viability of different blooming days （the day before flowering, day 1 , day 2, day 3, day 4, day 5, day 6, day 7, day 8） were determined by the 12-KI method and the TTC method, and the staining effect of the two staining methods on pollen viability were compared. I2-KI method was used to determine the pollen activity of Polygala tenuifolia pollens in different periods of time, different storage methods （wet preserved flowers and dry preserved flowers） and different storage conditions （4, 26, - 20, - 80 ℃） in different periods of time （2, 4, 8, 24, 48, 72 h）. And its stigma receptivity was estimated by the benzidine-hydrogen peroxide. Result： ①The pollen viability of P. tentdfolia decreased with the rise in the powder loosing time, the highest pollen viability was observed on the 4th day after flowering; ②from 8：00 to 18：00 during the daytime, the pollen viability between 10：00 and 16：00 was stronger;③dry pollen can prolong the storage time under the condition of -20 % ; ④ at the 5tb day after flowering, the stigma receptivity was the strongest. Conclusion： Pollen on the 4th day afterflowering can be collected, and artificial pollination can be adopted for stigma on the 5th day after flowering stigma artificial pollination, so as to provide a theoretical basis for breeding of P. tenuifolia.
作者
安衍茹
彭亮
孙涛
王媛媛
黄涛
赵停
张梅娟
张鸿琳
胡本祥
AN Yan-ru;PENG Liang;SUN Tao;WANG Yuan-yuan;HUANG Tao;ZHAO Ting;ZHANG Mei-juan;ZHANG Hong-lin;HU Ben-xiang(Shaanxi University of Chinese Medicine,Xianyang 712046,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2018年第17期42-46,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家中药标准化项目(ZYBZH-Y-QIN-36:202410001)
公益性中医药行业科研专项(201507002-1-08)
关键词
远志
花粉活力
柱头可授性
Polygala tenuifolia
pollen viability
stigma receptivity
