摘要
目的:建立大鼠被动拉伸实验模型,观察重复性和持续性被动拉伸训练后腓肠肌HGF、MGF及其基因表达的变化,研究不同拉伸方式在骨骼肌卫星细胞激活和增殖过程中的作用。方法:24只健康成年SD大鼠随机分为3组。对照组(N组,n=8)不做任何处理;对重复被动拉伸组(SR组,n=8)进行重复被动拉伸训练,2 s/次,15次/min,15 min/d,4次/周,共10周;对持续被动拉伸组(SC组,n=8)进行持续被动拉伸训练,15 min/d,4次/周,共10周。训练结束后取腓肠肌,测定腓肠肌湿重,ELISA法测定MGF、HGF,RT-PCR测定MGF、HGF的基因表达。结果:SC组和SR组实验后腓肠肌重量均上升,其中SR组与N组和SC组比较,差异具有统计学意义(P<0.01);在HGF、MGF及其m RNA表达水平上,SC组和SR组较N组均有上升,但SR组较SC组更加显著(P<0.01)。结论:持续性被动拉伸训练较重复性被动拉伸训练能够更有效激活肌卫星细胞进入细胞周期进行分裂增殖,实现肌纤维的修复和再生。
Objective： To examine the changes of the mRNA expression of HGF and MGF of rats＇ gastrocnemius after repetitively and continuously passive stretching through establishing the experimental model of passive stretching in rats, and investigate the effects of the passive stretching on the activation and proliferation of satellite cells of the skeletal muscles. Methods： 24 healthy adult SD rats were randomly divided into 3 groups. The control group（N group, n=8）was given no exercise; the repetitively-passive stretching group（SR group, n=8）with the right gastrocnemius muscles was stretched manually and repetitively for 2 seconds/time,15 times/minute, 15 minutes/day, 4 times/week and 10 weeks in total; the continuously-passive stretching group（SC group, n=8） with the right gastrocnemius muscles were stretched manually and continuously for 15 minutes/day, 4 times/week and 10 weeks in total. After training, their right gastrocnemius were taken out respectively to measure the wet weight; the HGF and MGF were measured by the ELISA method, and the expression of HGF mRNA and MGF mRNA in gastrocnemius muscles were measured by the RT-PCR.Results： After the passive stretching exercise,the weight of gastrocnemius muscles of both SC group and SR group significantly increased, and SR group increased much more obviously than the other two groups（P〈0.01）. Compared with N group, mRNA expression of HGF and MGF in SC group and SR group increased higher, but SC group was lower than SR group（P〈0.01）.Conclusion： Continuously passive stretching can activate the satellite cells to enter into the cell cycle of differentiation and proliferation more effectively than repetitively passive stretching. The restoration and regeneration of muscle fibers can be realized.
作者
吴国梁
李娜
Wu Guoliang;Li Na(College of Physical Education,Guangxi Science ＆ Technology Normal University,Liuzhou,Guangxi 545004,China)
出处
《大理大学学报》
CAS
2018年第8期25-29,共5页
Journal of Dali University
基金
广西省高校中青年教师基础能力提升项目(KY2016YB551)
