摘要
目的:分别将不同浓度的在位及异位子宫内膜细胞注射到裸鼠的腹部皮下,观察各组异位病灶的形成并进行比较分析,拟建立一种对造模鼠创伤小,且可连续、动态观察的可视化子宫内膜异位症(EMs)模型。方法:收集20例EMs患者的在位子宫内膜组织和异位病灶子宫内膜组织,分别消化培养子宫内膜细胞。将在位及异位子宫内膜细胞分别以2.5×10~6/200μL、5×10~6/200μL和1×10~7/200μL 3个浓度注射到裸鼠腹部皮下,观察并记录各组病灶开始出现的时间。在注射细胞后第5、10和15天分别计算各组病灶形成率和体积。第15天时取材,通过形态学分析和免疫组织化学方法鉴定病灶。通过比较,确定建立可视化EMs模型所需的最佳细胞种类、细胞浓度及病灶形成时间。结果:在裸鼠腹部皮下分别注射在位及异位子宫内膜细胞后均可成功形成可视化病灶。病理分析显示病灶中密集的间质细胞包绕着腺体,免疫组织化学鉴定显示病灶均由人子宫内膜细胞形成。各组病灶开始出现的时间差异均无统计学意义(F=0.230,P=0.942)。最佳注射细胞浓度为5×10~6/200μL,建立模型最佳观察时间是注射细胞后第10天。注射5×10~6/200μL浓度的细胞后在第10天时异位子宫内膜细胞组病灶发生率为100%,而在位子宫内膜细胞组病灶发生率为75%,2组之间差异有统计学意义(χ~2=9.143,P=0.002)。结论:异位的子宫内膜细胞比在位子宫内膜细胞更适合建立可视化EMs模型。将5×10~6/200μL异位子宫内膜细胞注射到裸鼠腹部皮下,在第10天时即可成功建立可视化EMs模型,成模率为100%,该病灶可以无创、连续、动态地观察,是研究腹壁EMs的理想模型。
Objective：Injecting different concentrations of human eutopic and ectopic endometrial cells into the abdominal subcutaneous tissue in nude mice. The formation of ectopic lesions was observed and compared, in order to establish a visible endometriosis model（EMs） that was non-invasively, continuously and dynamically. Methods：Twenty human eutopic endometrial samples and ovarian endometriosis cyst walls were obtained, endometrial cells were digested and cultured respectively. Three different concentrations of 2.5×10~6/200 μL, 5×10~6/200 μL and 1×10~7/200 μL were injected respectively into the abdominal subcutaneous tissue of nude mice. The time when lesions began to appear were observed and recorded. The incidence and volume of lesions on the 5 th day, 10 th day and 15 th day after injection was calculated. On the 15 th day after injection, the visible lesions were removed and identified by histologic and immunohistochemical examination. Comfirm the best cells, the optimal concentration of cells and the suitable time to observe the lesions through comparation. Results：Visible lesions could be successfully induced by injecting human eutopic and ectopic endometrial cells. Pathological analysis of lesions showed that glandular structures were surrounded by dense stromal cells. Immunohistochemical examination results confirmed that the lesions were formed by human endometrial cells. There were no statistically significant differences among the groups on the time when a lesion occurred（F=0.230, P=0.942）. The optimal concentration of injected cells was 5 ×10~6/200 μL, the best time to establish the model was on the 10 th day after injection. Under these conditions, the incidence of lesions on the 10 th day was 100% in the ectopic cells group and 75% in the eutopic cells group, the difference was statistically significant（ χ~2=9.143,P=0.002）. Conclusions：The ectopic endometrial cells were more suitable for establishing a visible EMs model compared with the eutopic endometrial cells. Co-cultured ectopic endometrial cells of 5 ×10~6/200 μL concentration were injected in the abdominal subcutaneous of nude mice could successfully establish the visible EMs model on the 10 th day and the incidence could up to 100%. The lesions could be observed non-invasively, continuously and dynamically. It was an ideal model to study the abdominal wall EMs.
作者
苏晓华
宋殿荣
张英
SU Xiao-hua;SONG Dian-rong;ZHANG Ying(Ninghe Hospital of Traditional Chinese Medicine,Tianjin 301500,China;The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300250,China;Tianjin Medical University,Tianjin 300070,China)
出处
《国际妇产科学杂志》
CAS
2018年第4期339-345,共7页
Journal of International Obstetrics and Gynecology
