摘要
建立了SPE-HPLC法同时测定3种大麻酚的方法。液相检测条件：Venusil XBP C18（L）：4.6×150 mm,5μm;流动相为V（甲醇）∶V（0.1%甲酸）=80∶20;柱温为40℃;检测波长为220 nm;流速为1 m L/min。3种大麻酚的线性回归方程R2均大于0.999,精密度实验、重复性实验RSD均在1.05%以内,回收率范围为99.6%-112.7%。此方法操作简便、准确度高,可作为3种大麻酚的质量控制标准。
To establish a SPE-HPLC method for three cannabinoids.The conditions of HPLC contain Venusil XBP C18（ 4. 6×150 mm,5 μm） column and the mobile phase with methanol of 80% and 0. 1% formic acid of 20%. The column temperature was40 ℃ and the cannabinoids were detected at 220 nm.The flow rate was 1 m L/min.The recoveries are greater than 0. 999.The RSD of precision and repeatability are less than 1. 05%.The recoveries are between 99. 6% and 112. 7%.This method is easy to operate and high accuracy,it can be used as quality control standards of three cannabinoids.
作者
张旭
孙宇峰
田媛
石雨
崔宝玉
高宝昌
ZHANG Xu;SUN Yu-feng;TIAN Yuan;SHI Yu;CUI Bao-yu;GAO Bao-chang(Daqing Branch of Heilongjiang Academy of Sciences, Daqing 163319, China)
出处
《化学试剂》
CAS
北大核心
2018年第6期547-550,554,共5页
Chemical Reagents
基金
黑龙江省科学院青年创新基金面上项目(CXMS2017DQ03)
国家国际科技合作专项项目(2015-DFR50260)
