摘要
目的探讨β-榄香烯对糖尿病性视网膜病变增生期大鼠视网膜的干预作用。设计实验研究。研究对象6周龄雄性GK大鼠30只。方法 30只GK大鼠随机分为对照组10只、模型组10只、β-榄香烯组10只,对照组置于正常大气环境下饲养,模型组、β-榄香烯组都建立了糖尿病性视网膜病变增生期模型,β-榄香烯组在模型建立后经玻璃体注射β-榄香烯1μl进行治疗。测定与记录各组治疗6周后a波峰潜时、视网膜铺片新生血管钟点数、无灌注区面积,检测TGF-β1与Fn蛋白表达水平。主要指标a波峰潜时、视网膜铺片新生血管钟点数、无灌注区面积,TGF-β1与Fn蛋白表达水平。结果模型组、β-榄香烯组都造模成功;对照组、模型组、β-榄香烯组实验后a波峰潜时分别为(19.44±1.59)ms、(23.40±1.42)ms和(21.49±1.94)ms,a波振幅分别为(225.20±66.23)Uv、(217.30±45.10)Uv和(233.10±54.01)Uv(P均<0.05)。对照组、模型组、β-榄香烯组实验后的视网膜铺片新生血管钟点数分别为8.01±1.24、3.67±0.82和6.14±1.11,无灌注区面积分别为(0.30±0.11)cm^3、(3.09±0.44)cm3和(0.29±0.13)cm^3(P均<0.05)。Western蛋白印迹法检测显示β-榄香烯组与模型组TGF-β1和Fn蛋白表达水平均比对照组明显低(P均<0.05),且β-榄香烯组明显高于模型组(P均=0.000)。结论β-榄香烯可减轻糖尿病性视网膜病变增生期大鼠的视网膜病变程度,抑制血管内皮细胞增生与抑制新生血管生成,改善视网膜功能。
Objective To investigate the effects of β-elemene on the retina of proliferative diabetic retinopathy in rats. Design Ex- perimental study. Participants 30 GK rats, 6 weeks old,males. Methods 30 GK rats were randomly divided into control group of 10 rats, model group of 10 rats, and elemene group of 10 rats. The rats of control group were feeded under normal atmospheric environ- ment; the proliferative diabetic retinopathy model were established in the rats of model group and β-elemene group, and the rats of β-elemene group were given β-elemene 1 txl intravitreal injection after the establishment of model. The peak latency of wave a in ERG, the number of new blood vessels and the area of no perfusion area at 6 weeks after treatment were measured and recorded, and the lev- els of TGF-β 1 and Fn protein were detected and recorded. Main Outcome Measures The peak latency of wave a in ERG, the number of new blood vessels and the area of no perfusion area, and the levels of TGF-β 1 and Fn protein. Results The proliferative diabetic retinopathy models were all successfully established in the model group and the β-elemene group. The a wave latency in the control group, model group and β-elemene group was （19.44±1.59）ms, （23.40±1.42）ms and （21.49±1.94）ms, respectively, and the a wave ampli- tude was （225.20±66.23）Uv, （217.30±45.10）uv and （233.10±54.01）Uv, respectively （all P〈0.05）. The retinal neovascularization hours in the control group, model group and β-elemene group as 8.01± 1.24, 3.67±0.82 and 6.14±1.11, and the non perfusion area was （0.30±0.l 1）cms, （3.09±0.44）cm3 and （0.29±0.13）cm3 （all P〈0.05）. Western blot analysis showed that the expression levels of Fn and TGF-β1 in the model group and β-elemene group were significantly lower than those in control group （P〈0.05）, and the expression levels in the [3-elemene group were significantly higher than that of model group （P=0.000, 0.000）. Conclusion β-elemene can improve the retinopathy of proliferative diabetic retinopathy rats, inhibiting the proliferation of vascular endothelial cells and angiogenesis, and it can improve the eIectrophysiological injury.
作者
代海燕
杨岚
朴天华
DAI Hai-yan;YANG Lan;PIAO Tian-huca(Hongqi Hospital, Mudanjiang Medical College, Mudanjiang 157000, Heilongjiang Province, China)
出处
《眼科》
CAS
CSCD
2018年第3期206-209,共4页
Ophthalmology in China
基金
黑龙江省卫生计生委科研课题(2016-386)
