摘要
过敏原风险问题已成为重要的食品安全问题,如何快速准确地检测出食物中的过敏原成为当前亟需解决的关键。目前常用的过敏原检测方法有免疫学检测、质谱及SPR等技术,但这些方法均有一定程度的局限性。通过引入分子生物学鉴定手段,建立小麦、榛子的过敏原基因数据库,并依据数据库寻找特异性序列并设计探针引物,建立一种快速、便捷、高效的过敏原检测方法,并适当调整了方法的检出限,降低了检测误判的风险。通过建立实时荧光PCR方法,可快速筛选样品的过敏原基因,简化了食品中过敏原成分的鉴定,降低了实验成本与技能要求,降低了检测的难度,缩短了实验时间。通过适用性验证与检出限验证实验,该方法用于过敏原成分鉴定可以达到理想的效果,重复性好,准确率高,检出限为1%。
Food allergies are in increasing issue in food safety. Therefore,the evaluation and detection of food allergens become more and more important. To quickly and accurately detect food allergens is an urgent need. At present,the most common method used in allergen detection are immunological detection,mass spectrometry and SPR technology. However,there are some limitations in these methods. We used molecular biology techniques and established a database of allergen genes for wheat and hazelnut,and designed primers by finding the specific sequences. In order to find a rapid,convenient and efficient method for the detection of allergen,we implemented a real time fluorescence PCR method for a rapid screening of the allergen gene. It is simple,low-cost,easy for operation and time saving. The detection limit was increased,and miss-dection rate was lowed. Through the applicability test and LOD experiments,we found the method is applicable for the identification of allergens,good repeatability and accuracy with the detecting limit of 1%.
作者
尚柯
张彪
段庆梓
张玉
王巍
梁恒兴
SHANG Ke;ZHANG Biao;DUAN Qing-zi;ZHANG Yu;WANG Wei;LIANG Heng-xing(Chengdu Institute for Food and Drug Control, Chengdu 610045, China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2018年第4期234-240,共7页
Food and Fermentation Industries
基金
成都市科技惠民应用示范项目(2015-HM02-00099-SF)
关键词
过敏原成分检测
实时荧光PCR
检出限
allergen detection
real-time fluorescence PCR
limit of determination （LOD）
