摘要
研究初步建立了NDM-1基因的实时荧光PCR快速检测鉴定方法。研究根据NDM-1序列,设计并合成了PCR检测引物(ND-T-F和ND-T-R)和探针(ND-T-PROBE),对NDM-1质粒DNA和其他11个标准菌株进行了实时荧光PCR检测。结果表明,携有NDM-1基因的质粒DNA模板都出现了很强的特异信号,而其他对照病菌均未出现特异荧光信号,证明这套引物及探针具有NDM-1基因检测的特异性。灵敏度测定结果,此体系可检出10fg/μL质粒DNA模板。研究表明,TaqMAN实时荧光PCR方法是一种特异、灵敏、快速的NDM-1基因检测方法。
NDM-1 was an important gene of superbacteria with broad-spectrum resistance to antibiotic.A TaqMAN realtime PCR method was developed to detect NDM-1gene.Specific primers and TaqMAN probe were designed based on the gene sequence in GeneBank.The probe used in this assay were shown to be specific for plasmid with NDM-1 and did not react with other 11 standard bacteria strains. This technique was sensitive with the detection limit of 10 fg/μL DNA.Real-time fluorescent PCR is a specific,sensitive,rapid and highly effective method for the detection and identification of NDM-1.
出处
《食品安全质量检测学报》
CAS
2011年第3期-,共5页
Journal of Food Safety and Quality
基金
山东检验检疫局科技专项:食品中超级细菌耐药基因NDM-1基因多重复合痕量检测体系的建立(SK2008-19)
