摘要
通过观察柴胡-白芍药对治疗抑郁模型大鼠海马中枢神经递质变化及BDNF和Trk B表达的情况,探讨其抗抑郁症的机理.采用HPLC法测定海马组织单胺类神经递质5-HT(5-羟色胺)、NE(去甲肾上腺素)、DA(多巴胺)的质量比.采用免疫组化法检测海马中BDNF(海马脑源性神经元)和Trk B(受体型酪氨酸蛋白激酶)阳性神经元面密度值的变化.结果表明,与模型组比较,柴胡白芍药对高、中剂量组海马单胺类神经递质5-HT,NE和DA质量比均显著增加,差异均有统计学意义(P<0.05或P<0.01);上调BDNF和Trk B阳性神经元面密度值(P<0.05或P<0.01).其作用机制可能是提高大鼠脑内海马组织和皮质组织NE、5-HT、DA质量比及上调BDNF(海马脑源性神经元)和Tr KB(受体型酪氨酸蛋白激酶)的表达.
To observe the changes of central neurotransmitter and the expression of BDNF and Trk B in the hippocampus of rats treated with Bupleurum and Paeonia lactiflora,and to explore the mechanism of its antidepressant. The levels of 5-HT（5-hydroxytryptamine）,NE（norepinephrine） and DA（dopamine） in hippocampus were measured by HPLC. The changes of BDNF（hippocampal brain-derived neurons） and Trk B（receptor tyrosine protein kinase） positive neurons in the hippocampus were detected by immunohistochemistry.Compared with the model group,the contents of 5-HT,NE and DA in the hippocampus mono-amines neurotransmitters were significantly increased in the high and middle dose groups,and the differences were statistically significant（P 0. 05 or P 0. 01）,and the uptake of BDNF and Trk B positive neurons was increased（P 0. 05 or P 0. 01）. The mechanism of action may be to improve the expression of NE,5-HT and DA in hippocampus and cortical tissue of rat brain and up-regulate the expression of BDNF（hippocampal brain-derived neurons） and Tr KB（receptor tyrosine protein kinase）.
作者
张洪财
王文姌
陈雁雁
ZHANG Hong-cai1, WANG Wen-ran2, CHEN Yan-yan2.(1.Research Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, China; 2. First Affliated Hospital, Heilongjiang University of Chinese Medicine, Harbin 150040, Chin)
出处
《哈尔滨商业大学学报（自然科学版）》
CAS
2017年第5期519-522,共4页
Journal of Harbin University of Commerce：Natural Sciences Edition
基金
黑龙江中医药大学校科研基金资助项目(201210)
关键词
柴胡-白芍药对
抑郁症
神经营养因子
单胺类神经递质
bupleurum - white peony drug pairs
depression
neurotrophic factor
monoamine neurotransmitters
