摘要
目的观察慢性温和不可预知性应激(CUMS)抑郁模型大鼠脑内单胺类神经递质水平的变化,揭示柴胡白芍药对的抗抑郁作用机制。方法采用CUMS制备大鼠抑郁模型,大鼠随机分为对照组,模型组,阳性药组(盐酸氟西汀15 mg/kg),柴胡白芍药对低、中、高剂量(生药8、16、32 g/kg,柴胡白芍配比1∶1)组,柴胡低、中、高剂量(生药8、16、32 g/kg)组,白芍低、中、高剂量(生药8、16、32 g/kg)组,各给药组连续ig给予治疗药物14 d。给药结束后取各组大鼠脑组织,采用HPLC法测定海马组织、皮质组织和下丘脑组织中单胺类神经递质去甲肾上腺素(NE)、五羟色胺(5-HT)、五羟吲哚乙酸(5-HIAA)水平的变化。结果与模型组比较,柴胡白芍药对高、中剂量组海马组织、皮质组织、下丘脑组织中NE和5-HT水平明显升高,5-HIAA水平均明显降低(P<0.05、0.01);柴胡白芍药对低剂量组、单用柴胡和单用白芍各剂量组可以不同程度地逆转NE和5-HT的减少和降低5-HIAA的水平(P<0.05)。结论柴胡白芍药对和单用柴胡或者单用白芍都可以通过调节大鼠脑内单胺类神经递质水平而起到抗抑郁的作用,柴胡白芍药对的抗抑郁作用优于单用柴胡或单用白芍。
Objective To observe the change of single amine neurotransmitter content in brain of rats, to reveal the antidepressant mechanism of herb pair of Bupleuri Radix and Paeoniae Alba Radix. Methods Chronic unpredictable mild stress （CUMS） depression model was successfully established on rats. Rats were randomly divided into control, model, positive drug （fluoxetine hydrochloride 15 mg/kg）, low-, mid-, and high-dose of herb pair of Bupleuri Radix and Paeoniae Alba Radix （crude drug of 8, 16, and 32 g/kg, Bupleuri Radix-Paeoniae Alba Radix ratio 1 ： 1）, low-, mid-, and high-dose of Bupleuri Radix （crude drug 8, 16, and 32 g/kg） group, and low-, mid-, and high-dose ofPaeoniae Alba Radix （crude drug 8, 16, and 32 g/kg） groups, which were ig given drugs for 14 d. After the administration, the brain tissue of rats was taken out. The monoamine neurotransmitter [norepinephrine （NE）, 5-hydroxytryptamine （5-HT）, and 5-hydroxyindoleacetic acid, （5-HIAA）] content changes of material could be detected by high performance liquid chromatography （HPLC）. Results Compaed with the model group, the contents of NE and 5-HT increased obviously, while the content of 5-HIAA decreased obviously in the hippocampus, cortex, and hypothalamus in the mid-, and high-dose of herb pair of Bupleuri Radix and Paeoniae Alba Radix group （P〈0.05, 0.01）; In the low-dose herb pair of Bupleurt Radix and Paeoniae Alba Radix groups, the decrease-contents of NE and 5-HT could be reversed of and the level of 5-HIAA could be decreased （P〈0.05） in a varying degrees. Conclusion The herb pair of Bupleuri Radix and Paeoniae Alba Radix, Bupleuri Radix, or Paeoniae Alba Radix could play the role of anti-depression by regulation of the manoamine neurotransmitter in the brain of rats. The anti-depressant effect of the herb pair of BupleuriRadix and Paeoniae Alba Radix is better than that of Bupleuri Radix or Paeoniae Alba Radix.
出处
《中草药》
CAS
CSCD
北大核心
2016年第16期2887-2892,共6页
Chinese Traditional and Herbal Drugs
基金
天津市科委自然科学基金重点项目(13JCZDJC31600)
关键词
柴胡
白芍
药对
单胺类神经递质
抑郁症
慢性温和不可预知性应激
去甲肾上腺素
五羟色胺
五羟吲哚乙酸
Bupleuri Radix
Paeoniae Alba Radix
herb pair
monoamine neurotransmitter
depression
chronic unpredictable mildstress depression
norepinephrine
5-hydroxytryptamine
5-hydroxyindoleacetic acid
