摘要
目的评价利用梅里埃VITEK 2 Compact全自动微生物分析系统对阳性血培养标本进行快速鉴定及药敏试验结果的可靠性及临床应用价值。方法收集2017年5月—8月福建中医药大学附属福鼎医院的124份BD Bactec 9120血培养仪报阳标本,其中革兰阳性(G+)球菌46株,革兰阴性(G^-)杆菌78株。用分离胶管以3500 r/min离心10 min,弃去上清液,取中间白色菌膜层配置0.5麦氏浊度菌液。根据直接涂片结果,选择合适的鉴定药敏卡片,用VITEK 2 Compact进行快速鉴定和药敏试验,以常规鉴定和药敏方法作为参照标准。结果与常规鉴定和药敏方法比较,以快速鉴定及药敏试验方法检测的G^-杆菌结果完全符合率为98.7%(1140/1 155),重大错误率为0.43%(5/1 155);G^+球菌完全符合率为98.4%(561/570),重大错误率为0.7%(4/570)。结论利用VITEK 2 Compact对血培养报阳标本进行快速鉴定及药敏试验的方法结果准确度较高,从血培养报阳到出具鉴定和药敏初步报告的时间约为8 h,大大缩短了用时,易于在临床上得到广泛使用。
Objective To evaluate the reliability and clinical applicability of rapid bacterial identification and antimicrobial susceptibility test results of using Biomerieux VITEK 2 Compact automatic microbial analysis system directly on positive blood culture specimens. Methods From May 2017 to August 2017, 124 positive samples were obtained from Fuding Affiliated Hospital of Fujian Traditional Chinese Medicine University. The samples were cultured by BD blood culture instrument, among which 46 strains were Gram-positive（G~＋） cocci and 78 strains were Gram-negative（G^-） bacillus. These strains were isolated by 3 500 r/min centrifuge for 10 min using serum separating tube, the supernatant was discarded and then the middle white bacterial membrane layer was collected to prepare bacterial fluid of 0.5 Mixwell turbidity. The appropriate identification antimicrobial susceptibility card was selected according to the result of direct smear, then the VITEK 2 Compact was used to make rapid identification and antimicrobial susceptibility test, and routine identification and antimicrobial susceptibility methods were used as the reference standard as well. Results Compared with the routine identification and antimicrobial susceptibility methods, the rapid identification and antimicrobial susceptibility test methods showed that the complete coincidence rate of Gram-negative（G^-） bacillus was 98.7%（1 140/1 155） and the significant error rate was 0.43%（5/1 155）; the complete coincidence rate of Gram-positive（G~＋） cocci was 98.4%（561/570） and the significant error rate was 0.7%（4/570）. Conclusions The accuracy of using VITEK 2 Compact to make direct identification and rapid antimicrobial susceptibility test on positive blood culture specimens is relatively high. The time from the moment of reporting positive result of blood culture to presenting bacterial identification and preliminary antimicrobial susceptibility test report is about 8 h, greatly shortening the corresponding time spent in conventional method, thus this method is easily applied widely in clinical practice.
作者
张袁露
陈娟
李步任
Zhang Yuanlu, Chen Juan, Li Buren.(Department of Clinical Laboratory, the Affiliated Fuding Hospital of Fujian University of Traditional Chinese Medicine, Fuding 355200, Fufian, Chin)
出处
《实用检验医师杂志》
2018年第1期24-26,共3页
Chinese Journal of Clinical Pathologist
关键词
阳性血培养
VITEK
2
COMPACT
快速鉴定
药敏试验
Positive blood cultures
VITEK 2 Compact
Rapid identification
Antimicrobial susceptibility test
