摘要
目的:建立同时测定乌灵菌粉中腺苷、染料木素、5-甲基蜂蜜曲霉素含量的HPLC方法。方法:Pntulips~@BP-C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相:乙腈-0.1%甲酸溶液,梯度洗脱;流速:1.0 ml·min^(-1);柱温:35℃,检测波长:254 nm。结果:腺苷在0.162~1.62μg范围内线性关系良好(r=0.999 2),平均加样回收率为98.12%,RSD为1.32%(n=6);染料木素在0.02~0.2μg范围内线性关系良好(r=0.999 9),平均加样回收率为98.89%,RSD为1.10%(n=6);5-甲基蜂蜜曲霉素在0.022~0.22μg范围内线性关系良好(r=0.999 9),平均加样回收率为99.87%,RSD为0.98%(n=6)。结论:该方法可同时测定腺苷、染料木素、5-甲基蜂蜜曲霉素的含量,可为乌灵菌粉的质量控制及后期研究提供基础。
Objective： To establish a HPLC method for the simultaneous determination of adenosine,genistein and 5-hydroxymellein in Xylarin nigripes powder. Methods： The chromatographic separation was carried out on a Pntulips@BP-C18 column（ 250 mm ×4. 6 mm,5 μm） at 35℃. The mobile phase consisted of acetonitrile-0. 1% formic acid with gradient elution at a flow rate of 1. 0 ml·min^-1,and the detection wavelength was 254 nm. Results： The linear range of adenosine was 0. 162-1. 62 μg（ r = 0. 999 2）. The average recovery was 98. 12% and the RSD was 1. 32%（ n = 6）. The linear range of genistein was 0. 02-0. 2 μg（ r = 0. 999 9）. The average recovery was 98. 89% and the RSD was 1. 10%（ n = 6）. The linear range of 5-hydroxymellein was 0. 022-0. 22 μg（ r =0. 999 9）. The average recovery was 99. 87% and the RSD was 0. 98%（ n = 6）. Conclusion： The method can be used to determine the contents of adenosine,genistein and 5-hydroxymellein,which provides the basis for the quality control and the further research of Xylaria nigripes.
出处
《中国药师》
CAS
2018年第3期527-529,共3页
China Pharmacist
