摘要
目的:建立了迷迭香水溶性成分的毛细管电泳特征图谱。方法:以20 mmol.L-1硼砂(pH=9.50)为背景电解质溶液,运行电压12 kV,紫外检测波长300 nm,重力进样时间5 s(高度10 cm),对不同产地迷迭香进行检测。结果:按电泳峰共有率fi≥90%为依据,确定10个不同产地迷迭香的毛细管电泳指纹峰为11个,各产地迷迭香的毛细管电泳特征图谱与对照毛细管电泳特征图谱的相似度较好。在不同时间对样品进行测定,各特征峰的相对迁移时间的RSD小于5.0%,相对峰面积的RSD在2.7%～8.0%之间,结果表明样品在36 h内稳定。结论:该方法简便准确,为迷迭香的质量控制提供了有效的手段。
Objective：To establish the specific chromatogram of water- soluble constituents of rosmarinus officina- lis by capillary eleetrophoresis. Methods： Twenty mmol· L^- 1 borax （ pH = 9.50） was used as background electro- lyte, and operation voltage of 12 kV was applied. UV - detection was adopted at the wavelength of 300 nm. Samples were introduced at a height of 10 cm for 5 s. Result： Samples of rosmarinus officinalis from 10 different origins were selected for analysis. Based on the value of rate of common electrophoretie peaks f, ≥90%, a set of eleetrophoretic fingerprint atlas of 11 peaks was determined for flavones in rosmarinus officinalis samples from 10 different areas. Good similarities between the eleetrophoretic specific chromatogram of the samples and the standards were obtained. Relative standard deviation among the values of relative migration times of various specific peaks obtained at differ- ent time points of sample introduction was less than 5.0% , and values of RSD＇s for the relative peak areas were in the range from 2.7% to 8.0%. The results obtained proved that the sample was stable within 36 h. Conclusion：The method is simple and accurate .which could be an effective method fur tha quality control of rosmarinus officinalis.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2013年第2期331-334,共4页
Chinese Journal of Pharmaceutical Analysis
基金
广西自然科学基金项目(桂科自0640038)
广西科学研究与技术开发技术项目(桂科能0992028-13)资助
关键词
毛细管电泳
相似度
迷迭香
特征图谱
迷迭香酸
capillary electrophoresis
similarity
rosmarinus officinalis
specific chromatogram rosemary acid
