摘要
为描述鸡肉产品中四环素耐药菌的污染状况,初步评价四环素耐药基因通过市售鸡肉产品进行迁移的潜在风险。在上海13个区采集55个冷冻鸡肉样品、50个冷鲜鸡肉样品和3个现宰鸡样品,分离对四环素或强力霉素不敏感的细菌(Tet^(is)或Dox^(is)),研究其耐药特征,并筛查7种四环素耐药基因和一类整合酶基因int I。结果表明,所有样品均分离出Tet^(is)菌,菌落数为1.00×103~1.51×108CFU/g;从96.19%的样品中分离出Dox^(is)菌,菌落数为5.01×10~7.76×106CFU/g;两类抗生素不敏感菌的数量在现宰样中最高,冷鲜样次之,冷冻样最低。738株Tet^(is)和Dox^(is)菌普遍对多种抗生素不敏感,其中165株菌中耐药基因tet A,tet B,tet C,tet E,tet G,tet L,tet M和int I的检出率分别为9.70%,20.00%,4.24%,9.70%,15.80%,29.70%,61.80%和46.10%。耐药基因的寄主菌包括埃希氏菌、葡萄球菌、肠球菌、奇异变形杆菌、不动杆菌、鞘氨醇杆菌和土地杆菌等,大多数菌的四环素最小抑菌质量浓度大于64μg/m L。综上,市售鸡肉产品中四环素耐药菌污染较为严重,可能包含巨大的耐药基因池,存在潜在的迁移风险。
In order to investigate the contamination of tetracycline resistant bacteria and the potential risk of transmitting antibiotic resistance（AR） encoding genes in retail chicken products, Tetracycline-and Doxycycline-insensitivity（Tet^is and Dox^is） bacteria were isolated from 55 frozen, 50 cold fresh and three slaughtered chicken products which purchased from 13 districts in Shanghai. The positive rates of Tet^is and Dox^is bacteria were 100% and 96.19%, and the prevalence ranged from 1.00×10^3 CFU/g to 1.51×10^8 CFU/g and 5.01×10 CFU/g to 7.76×10^6 CFU/g, respectively. The levels of these antibiotic insensitivity bacteria were higher in slaughtered samples than cold fresh and frozen samples normally.Multi-drug insensitivity was common, and the positive rates of AR genes of tet A, tet B, tet C, tet E, tet G, tet L, tet M and integrase gene int I were 9.70%, 20.00%, 4.24%, 9.70%, 15.80%, 29.70%, 61.80% and 46.102%, respectively.Escherichia sp., Staphylococcus sp., Enterococcus sp., Proteus mirabilis, Acinetobacter sp., Sphingobacterium sp. and Pedobacter sp. were the identified hosts for AR genes. Furthermore, the minimum inhibitory concentration（MIC） of Tet for most isolation was higher than 64 μg/m L. In conclusion, the contamination of tetracycline resistant bacteria was existent and a huge AR gene pool might be harbored in retail chicken products, which could be a potential avenue transmitted antibiotic resistant bacteria and AR genes.
作者
邵毅
李姝
黄柳娟
周昌艳
姚春霞
王华
Shao Yi;Li Shu;Huang Liujuan;Zhou Changyan;Yao Chunxia;Wang Hua(Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403;College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306;Shanghai Co-Elite Agro-Food Testing Service Co., Ltd., Shanghai 201106;Department of Food Science and Technology, The Ohio State University, Columbus 43210, Ohio;Department of Microbiology, School of Life Sciences, Hehai University, Shanghai 200433;Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai 201403)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2018年第1期250-256,共7页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金-青年科学基金项目(31401599)
上海市科技兴农重点攻关项目(应用基础类)(沪农科攻字(2014)第7-3-6号)
上海市青年科技英才扬帆计划项目(14YF1413000)
美国外交部国际教育协会美英联合全球创新倡议(GII)项目(GRT00033913)
关键词
四环素耐药菌
四环素耐药基因
鸡肉
tetracycline resistant bacteria
tetracycline resistance genes
chicken
