摘要
为明确耐甲氧西林金黄色葡萄球菌(MRSA)在鸡场的污染现状,提供其在人禽间传播的基础数据,采用FA-Ⅰ撞击式采样器采集鸡舍内空气中总细菌、金黄色葡萄球菌和MRSA,并采集同日龄鸡粪样本。分别考察空气和粪便中相应细菌浓度,分析其浓度随日龄变化趋势及其可能的影响因素。结果表明:空气和粪便中MRSA浓度平均值分别为(4.31×10~2±4.56×10~2)CFU·m^(-3)和(3.37×10~4±4.83×10~4)CFU·m^(-3),占金黄色葡萄球菌比例分别为41.99%±26.99%和22.11%±20.06%。随日龄增加,鸡舍内空气和粪便中总细菌浓度呈线性增加;金黄色葡萄球菌及MRSA浓度整体上随日龄增加而升高,同时受到温度和舍内颗粒物浓度变化影响。PM_(2.5)负载MRSA浓度的变化规律与空气中总MRSA相似,均值为(1.51×10~2±1.7×10~2)CFU·m^(-3),占总MRSA的38.13%±20.17%。
In order to provide base data on the pollution status of methicillin-resistant Staphylococcus aureus（MRSA）in poultry farms and reveal its transmission between humans and livestock, in this research, an FA-Ⅰ sampler was used to collect total bacteria, Staphylococcus aureus, and methicillin-resistant MRSA in the air layer, and feces samples were collected at the same time. In order to analyze the variation trend and its possible influencing factors as a function of time（days）, the concentrations of bacteria in the air and the corresponding feces were examined. The results showed that the average concentrations of MRSA in the air and feces were（4.31×10^2±4.56×10^2）CFU·m^-3 and（3.37×10^4±4.83×10^4）CFU·m^-3, respectively, with ratios in the Staphylococcus aureus of 41.99% ±26.99% and 22.11% ±20.06% . The total bacteria concentration increased linearly with increasing age in both the air and feces samples. The concentration of Staphylococcus aureus and MRSA in the air increased with time and was affected by both temperature and particle concentration. The variation of MRSA concentra-tion in PM2.5 was similar to that of total MRSA in the air. The average concentration of the PM2.5 portion as（1.51×10^2±1.7×10^2）CFU·m^-3,which accounted for 38.13% ±20.17% of the total MRSA.
作者
韩帅
孙兴滨
陈默
高敏
王旭明
HAN Shuai;SUN Xing-bin;CHEN Mo;GAO Min;WANG Xu-ming(l.College of Forestry Science, Northeast Forestry University, Harbin 150040, China;Beijing Agro-Biotechnology Research Center, Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;College of Biologieal Sciences, China Agrieultural University, Beijing 100193, China)
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2018年第1期148-156,共9页
Journal of Agro-Environment Science
基金
家禽产业技术体系北京市创新团队专项资金(BAIC04-2017)
北京市农林科学院科技创新能力建设专项(KJCX20151204)
北京市科技新星(Z151100000315058)
北京市优秀人才(2014000020060G178)
关键词
耐甲氧西林金黄色葡萄球菌
生物气溶胶
细颗粒物
蛋鸡
影响因素
methieillin-resistant Staphylococcus aureus （ MRSA ）
bioaerosol
fine particulate matter
layer
influencing factors
