摘要
目的观察健脾益气摄血方及其拆方对辛伐他汀诱导斑马鱼心脏出血模型血流量的改善作用,探索不同治则对斑马鱼血流动力学的变化联系。方法将660尾72 h受精卵黑色素等位基因突变型Albino品系斑马鱼随机分为正常对照组、模型对照组和实验药物A(健脾颗粒配方)、B(健脾益气颗粒配方)、C(健脾益气补血颗粒配方)、D(健脾益气补血摄血颗粒配方)组。每种药物设100、250、500、750、1000μg/m L五个不同浓度组,每组30尾。先以A、B、C、D组不同浓度药物处理斑马鱼4 h,再用50μmol/L辛伐他汀处理30 min,用心跳血流分析系统采集各组20尾斑马鱼血流影像,分析血流量,计算改善率。结果(1)与模型对照组比较,A、B组各药物浓度组对辛伐他汀诱导的斑马鱼心脏出血模型的血流量改善均无效果;C、D组的500、750、1000μg/m L药物浓度均能提高斑马鱼出血模型血流量改善率(P<0.05)。(2)在改善辛伐他汀诱导斑马鱼心脏出血模型血流速度和血流量改善率方面,健脾益气补血摄血方1000μg/m L优于健脾益气补血方。结论健脾益气补血摄血治则综合运用能够明显提高辛伐他汀诱导斑马鱼心脏出血模型血流量改善率,体现了"气为血之帅,血为气之母"的气血相关理论。
Objective To observe the effect of Invigorating Spleen, Benefiting Qi, Controlling Blood Formula on the blood flow of Simvastatin-induced zebrafish cardiac hemorrhage model and to explore the relationship among the hemodynamic changes of zebrafish by different treatment. Methods The fertilized egg of 660 72-hour Albino zebrafish in melanin allele mutation was randomly divided into the normal control group, the model control group and group A （Invigorating Spleen Granule Formulations）, B （Invigorating Spleen, Benefiting Qi Granule Formulations）, C （Invigorating Spleen, Benefiting Qi, Replenishing Blood Granule Formulations）, D （Invigorating Spleen, Benefiting Qi, Replenishing Blood, Controlling Blood Granule Formulations） with different concentrations （100, 250, 500, 750, 1000 p,g/mL） of experimen- tal drugs. Each group of 30. First, the zebrafish of group A, B, C, D were treated with different concentrations of drugs for 4 hours, and then with 50 μmol/L Simvastatin for 30 minutes. Next, through collecting blood flow imaging of the 20 zebrafish in each group by heartbeat blood flow analysis system, the blood flow was analyzed and the improvement rate was calculated. Results ①Compared with the model control group, different concentrations of drug groups in group A, B had no effects on the improvement of blood flow in Simvastatin-induced zebrafish hemorrhage model; however, 500,750,1000 μg/mL of drugs in group C, D could significantly increase the improvement rate of blood flow in zebrafish hemorrhage model （P 〈 0.05）. ②In ameliorating the model＇s velocity of blood flow and its im- provement rate, 1000 μg/mL of Invigorating Spleen, Benefiting Qi, Replenishing Blood, Controlling Blood Formula was superior to that of Invigorating Spleen, Benefiting Qi, Replenishing Blood Formula. Conclusion The com- prehensive application of invigorating spleen, benefiting qi, replenishing blood, controlling blood can ameliorate the blood flow of Simvastatin-induced zebrafish cardiac hemorrhage model, which reflects the theory of ＂qi is the commander of blood and blood is the mother of qi＂.
出处
《中国医药导报》
CAS
2017年第36期9-12,24,共5页
China Medical Herald
基金
国家自然科学基金青年项目(81703903)
国家重点基础研究发展计划(973计划)课题(2013CB531705)
关键词
健脾益气摄血方
斑马鱼
心脏出血
血流量
Invigorating Spleen, Benefiting Qi, Controlling Blood Formula
Zebrafish
Cardiac hemorrhage
Blood flow
