摘要
目的建立人血清中硬脂酸(C18∶0)、油酸(C18∶1)、亚油酸(C18∶2)、十八碳三烯酸(C18∶3)、二十碳四烯酸(C20∶4)、二十碳五烯酸(EPA,C20∶5)和二十二碳六烯酸(C22∶6)7种饱和及不饱和脂肪酸的气相色谱测定方法。方法人血清中的7种脂肪酸经10%硫酸-甲醇在60℃水浴30 min,甲酯化。取出放冷至室温,加入正己烷和饱和氯化钠溶液,旋涡混匀,离心后取上层正己烷相,用气相色谱法测定,用相应的脂肪酸甲酯定性,外标法定量。结果在优化好的色谱条件下,7种脂肪酸甲酯化后可完全分离,重复性好,在5.0 mg/L^100 mg/L内相关系数(r)>0.999 4,检出限≤1.2 mg/L,10 mg/L、20 mg/L、50 mg/L 3个浓度的加标平均回收率为86.7%~93.6%,RSD为1.3%~3.2%。结论本方法前处理简单,定量准确,适用于人血清中饱和与不饱和脂肪酸的同时测定。
Objective To establish a method for the determination of seven saturated and unsaturated fatty acids,including stearic acid（ C18∶0）,oleic acid（ C18∶1）,lenoleic acid（ C18∶2）,linolenic acid（ C18∶3）,arachidonic acid（ C20∶4）,eicosapentaenoic acid（ C20∶5） and docosahexaenoic acid（ C22∶6）,in human serum by gas chromatography. Methods Seven fatty acids in human serum were esterified by 10% H2 SO4-CH3 OH at 60 ℃ for 30 min,then it was cooled to room temperature. n-hexane and saturated sodium chloride solution were added to the mixture,after centrifuging,the upper organic layer was analyzed by gas chromatography. The corresponding fatty acid methyl esters were used for qualitative analysis and external standard method was used for quantitative analysis. Results After esterification,seven fatty methyl ester could be baselinely separated under the optimized chromatographic conditions,with good reproducibility. The linearity of the method was within5. 0 mg/L-100 mg/L（ r 〉0. 999 4）,with LOD ≤1. 2 mg/L. The average recovery rate spiked at 10 mg/L,20 mg/L,50 mg/L were within 86. 7%-93. 6%,with RSDs of 1. 3%-3. 2%. Conclusion This method is simple and accurate,it can be used to determine the saturated and unsaturated fatty acids in human serum.
出处
《中国卫生检验杂志》
CAS
2017年第21期3058-3060,3064,共4页
Chinese Journal of Health Laboratory Technology
基金
浙江省自然科学基金(LY13B020002)
浙江省教育厅科研项目(Y201534630)
关键词
血清
脂肪酸
气相色谱法
Serum
Fatty acid
Gas chromatography
