摘要
为探讨毛竹Phyllostachys edulis笋竹快速生长期可溶性糖质量分数变化与PeTPS1和Pe SnRK1基因的表达情况,明确它们与毛竹快速生长的关系,采用实时荧光定量聚合酶链式反应(q RT-PCR)分析技术对笋竹快速生长期不同时间(黄昏后0,4和8 h)和不同部位(笋竹上部、中部、下部和竹篼)PeTPS1和Pe SnRK1基因表达量进行分析,并采用试剂盒法测定糖质量分数。结果表明:笋竹茎秆上部可溶性糖质量分数变化不显著;黄昏后8 h中部葡萄糖、果糖、蔗糖质量分数与黄昏时相比分别下降了2.2倍、1.4倍和1.6倍;营养储存器官竹蔸中黄昏后8 h葡萄糖、果糖、蔗糖、海藻糖质量分数与黄昏时相比分别下降了1.6倍、1.3倍、1.4倍和1.3倍。笋竹中部黄昏后8 h PeTPS1基因的表达量为下部的4.8倍;黄昏后8 h竹蔸中Pe SnRK1基因表达量均显著高于其他部位,为黄昏时的1.7倍。随黄昏时间变化毛竹快速生长过程中不同部位碳水化合物质量分数及其相关调控基因不断变化,可溶性糖不断消耗以供应笋竹快速生长部位的生长,同时PeTPS1基因被上调合成海藻糖以保证充足的碳源,而Pe SnRK1基因则呈现出与PeTPS1基因表达相反的变化趋势。推测T6P/SnRK1信号共同调节黑暗中笋竹快速生长,在毛竹快速生长这一关键生理过程中具有重要作用。研究成果为进一步明确毛竹速生生长机制以及指导其他树木的速生和育种具有重要意义。
To disc uss expression of the PeTPS1/PeSnRK1 gene and soluble sugar mass fraction in different parts of Phyllostachys edulis bamboo during the process of rapid growth and to clarify their relationship with this rapid growth, the relative expression of PeTPS1 and PeSnRK1 in the bamboo stump and the lower, middle,and upper parts of the Ph. edulis bamboo shoot were analyzed at sunset（0 h）, 4 h, and 8 h after sunset using the quantitative reverse-transcriptase polymerase chain reaction（q RT-PCR） analysis technique. Also, the mass fraction of soluble sugars was analyzed by the kit method. Results showed that the soluble sugar mass fraction in the upper part of the bamboo shoot did not change. The mid-section of the bamboo shoot mass fraction at 8 h after dusk compared to sunset was lower 2.2 times for glucose, 1.4 times for fructose, and 1.6 times for sucrose.Nutrient storage mass fractions in the bamboo stump at 8 h after sunset compared to 8 h after sunset was lower for glucose（1.6 times）, fructose（1.3 times）, sucrose（1.4 times）, and trehalose（1.3 times）. Expression of the PeTPS1 gene in the middle of the bamboo shoot was 4.8 times lower than in the lower part of the bamboo shoot. Expression of the PeSnRK1 gene in the bamboo stump compared to the other parts was higher at 8 h after sunset and was 1.7 times higher compared to dusk. These results could provide new insights into the mechanism of rapid growth of Ph. edulis as well as direct fast-growth and breeding of other trees.
出处
《浙江农林大学学报》
CAS
CSCD
北大核心
2017年第6期1016-1023,共8页
Journal of Zhejiang A&F University
基金
国家自然科学基金资助项目(31570686
30972397)
