摘要
为了分析马铃薯扩展蛋白基因家族的进化与功能,鉴定了马铃薯的扩展蛋白基因家族,分析了其基因结构特征、系统发育及表达模式等。结果表明,马铃薯基因组包含33个扩展蛋白基因,包括A(21)、B(5)、LA(1)和LB(6)4个亚家族,分布在马铃薯11条染色体上。扩展蛋白氨基酸长度为194~488 aa,编码蛋白质具有保守的结构域,蛋白质亚细胞定位预测结果表明所有蛋白均定位于细胞外。基因结构分析表明,马铃薯扩展蛋白家族有23个基因(69.7%)含有2~3个内含子。基因表达分析发现,该家族基因在马铃薯根、茎、匍匐茎、果实及块茎发育过程中表达丰度较高,并且其表达受到高温、盐、激素及病害等逆境的调控。
As an important component of plant cell wall,expansins have effects on loosening and increasing the flexibility of cell wall,and are thought to have function in many process of plant growth. To study potential function of expansin gene family in potato,we investigated expansin gene family with the whole genome database to analyse its structure,phylogenetic relationship and expression profiles. Potato genome contained 33 expansin genes which belonged to 4 subfamilies（ EXPA（ 21）,EXPB（ 5）,EXLA（ 1） and EXLB（ 6）） located on 11 different chromosomes. Expansin proteins ranged from 194 aa to 488 aa in size and had conserved domains. All 33 Expansin proteins were predicted to localize in extracellular. Gene structure analysis revealed that seventy percent（ 23/33） expansin genes had 2-3 introns. Transcriptome analysis showed that the potato expansin genes expressed highly in root,stem,stolon,mature fruit and tuber.
作者
理向阳
代丹丹
杨铁钢
郝西
LI Xiangyang DAI Dandan YANG Tiegang HAO Xi(Industrial Crop Institute, Henan Academy of Agricultural Sciences,Zhengzhou 450002,Chin)
出处
《华北农学报》
CSCD
北大核心
2017年第5期37-44,共8页
Acta Agriculturae Boreali-Sinica
基金
河南省农业科学院自主创新项目(2016ZZ20)
