摘要
采用单因素实验结合Box-Behnken中心组合设计方法,以蛋白质脱除率和综合指数为指标,考察木瓜蛋白酶浓度、酶解温度和酶解时间对黑根霉胞外多糖酶-Sevag法脱蛋白工艺的影响。经过响应面实验优化,确定最优条件为木瓜蛋白酶浓度425 U/mL、酶解温度62℃、酶解时间90 min,经三次重复实验验证,蛋白质脱除率与综合指数均值分别为54.08%和2.07。
The effects of papain concentration,enzymolysis temperature and time on the deproteinization of extracellular polysaccharide from Rhizopusnigricans in Enzyme-Sevag process were investigated by single-factor experiment combining with central composite design method of Box-Behnken.The optimal process parameters were determined based on the deproteinization rate and comprehensive index.After optimizing by response surface methodology,the optimal process parameters were confirmed as follows：papain concentration of 425 U/mL,enzymolysis temperature of 62 ℃ and time of 90 min.Following repeated experiments three times,the average deproteinization rateand comprehensive index were determined as 54.08 % and 2.07,respectively.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2017年第10期1773-1778,1784,共7页
Natural Product Research and Development
基金
安徽高校自然科学研究项目(KJ2016A723
KJ2015A199
KJ2017A257)
安徽省自然科学基金(1508085M H191)
皖南医学院中青年科研基金(WK201517
WK201601
WK201610)
大学生创新创业训练计划(201510368017
201510368119
201610368120)
关键词
黑根霉
胞外多糖
脱蛋白
酶-Sevag法
响应面法
Rhizopusnigricans exopolysaccharides deproteinization enzyme-sevag process response surface methodology
