摘要
以生物量和胞外多糖产量为指标,采用单因素和正交试验对摇瓶培养的拟康氏木霉(Tricho-dermapseudokoning)的液态发酵条件进行优化。优化后的拟康氏木霉发酵控制参数为:最佳培养基配方为麦麸30g/L,玉米粉30g/L,葡萄糖7.5g/L,KH2PO41g/L;发酵培养时间为6d;培养基的初始pH值为5.0～6.0;发酵温度为30±1℃。最佳培养条件下菌丝干重及胞外多糖的产量分别5.272g/L、0.622g/L。
The liquid fermentative condition of Trichoderma pseudokoning was studied through single factor and orthogonal design tests. The experimental results showed the optimized fermentative conditions are as follows： Culture medium formula is wheat bran 30g/L, corn meal 30g/L, sucrose 7.5g/L,KH2PO4 1g/L. The optimum fermentation period is 6d. The fermentation temperature and the initial pH-value of culture medium is 30±1℃ and 5.0-6.0 respectively. The mycelial dry weigh and the extracellular polysaccharides of Trichoderma pseudokoningii reached 5.272 g/L,0.622 g/L under the the optimized fermentative conditions.
出处
《中国农学通报》
CSCD
2006年第10期58-61,共4页
Chinese Agricultural Science Bulletin
基金
国家863计划资助项目(2002AA244031)
关键词
培养条件优化
菌丝体
胞外多糖
正交试验
Culture condition optimization, mycelia, extracellular polysaccharides, Orthogonal design experiment
