摘要
建立了水产品中21种磺胺类(SAs)和12种喹诺酮类(QNs)药物残留的超高效液相色谱-四级杆/静电场轨道阱高分辨质谱(UPLC-Q-Orbitrap HRMS)多残留检测方法。样品经过乙腈提取,乙腈饱和正己烷脱脂,内标法定量。结果表明,33种目标化合物在质量浓度为0.1~100ng/mL时线性关系良好,r^2≥0.996 0,定量限(LQD)为2μg/kg。平均加标回收率为54.3%~120.4%,相对标准偏差(RSDs)为1.7%~11.9%。该方法操作简便、灵敏度高、特异性强,可以满足日常检测要求。
An Ultra-High Performance l.iquid Chromatography-Qua-drupole/Electrostatic Field Orbitrap High-resolution Mass Spectrom etry method （UPLC Q-Orbitrap HRMS）has been developed for the determinations of the drug residues of 21 SAs and 12 QNs in aquatic products. The sample was extracted by acetonitrile, cleaned up by acetonitrile saturated normal hexane and quantified in internal stand ard method. The results showed that 33 products presented a good linear relation in the range of 0.1-100 ng/mL, r2〉0. 996. The limits for qualitative detection was 2 μg/kg. The average recoveries at three spike levels ranged were 54.3% -120.4%, with RSDs ran ging 1.7%-11.9%. The developed method was easy operation high sensitivity, which can meet the requirements of daily inspection.
出处
《食品与机械》
CSCD
北大核心
2017年第8期38-43,共6页
Food and Machinery
基金
国家质检总局科技计划项目(编号:2015IK260)
广东省省级科技计划项目(编号:2014A040401011
2015A050502010)
中山市社会公益重大专项(编号:2015B2295)
关键词
高分辨质谱
水产品
磺胺类
喹诺酮类
high-resolution mass spectroscopy
aquatic products
sul fonamides
quinlones
