摘要
红豆越橘是一种新兴水果,具有较高的营养及食疗药用价值。本研究利用高通量测序技术对野生红豆越橘的幼叶转录组进行了测序,并对测序数据进行生物信息学分析。总共获得大约2 G的纯净数据,拼装了48 736条Unigenes。GO数据库注释到的Unigenes分别涉及到生物学过程、细胞成分及分子功能相关的共43种生理代谢功能;18 728条Unigenes能被KOG数据库注释,共涉及25条代谢通路;8 321条Unigenes能被KEGG数据库成功注释,共涉及到5个功能大类、20个功能中类、123条代谢通路;30 467条Unigenes可被Nr数据库注释;21 551条Unigenes可被Swissprot数据库注释;以上4个数据库共注释到30 545条Unigenes,占全部Unigenes的62.67%;被以上4个数据库均注释到的Unigene为6 075条,占全部Unigenes的12.47%。同时,生物信息学分析还显示:全部Unigenes中,有5 197条Unigenes编码转录因子,涉及57个家族;2 747条Unigenes编码抗性基因,涉及19个家族。共检测到8 099个SSR位点,其中2碱基重复的SSR位点达5 791个,占SSR位点总数的71.52%。以上研究结果对了解红果越橘的生长发育、生理生化等分子机制,对进一步进行红豆越橘分子方面的相关研究均具有较强的参考价值。
Lingonberry（Vaccinium-vitis idaea） is a kind of new developing fruit,which has high nutritional,therapeutic and medical value.In this study,the transcriptome of lingonberry spires was sequenced by high throughput sequencing technology,and the sequencing data were analyzed by Bioinformatics.The results showed that totally about 2G clean reads were obtained,which could be assembled into 48 736 unigenes.The Unigenes annotated by the GO database involved 43 physiological and metabolic functions related to biological processes,cellular components and molecular functions;18 728 Unigenes could be annotated by KOG dataset,which were involved in 25 metabolism pathways;8 321 Unigenes could be annotated by KEGG dataset,which were involved in 5 major functional categories,20 middle functional categories,and 123 metabolism pathways;30 467 Unigenes could be annotated by Nr dataset,21 551 unigenes could be annotated by Swissprot dataset;the above 4 databases were annotated to 30 545 Unigenes,accounting for 62.67% of all Unigenes,and 6 075 Unigene accounting for12.47% of the total Unigenes could be annotated by all above 4 database.Meanwhile,bioinformatic analysis found that there were 5 197 Unigenes coding transcription factors in all Unigenes,involving 57 families,and 2 747 Unigenes coding resistance genes and involved 19 families;A total of 8 099 SSR loci were detected among all Unigenes,of which 5 791 SSR loci had 2 base repeated,accounting for 71.52% of total SSR loci.The research results could be helpful to understand the molecular mechanisms of growth and development,physiology and biochemistry of lingonberry,and had a stronger reference value for further studies on the molecular aspects of lingonberry.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2017年第9期3871-3881,共11页
Genomics and Applied Biology
基金
贵州省教育厅重点项目(黔教合KY字(2013)186)
贵州省科技重大专项(黔科合重大专项字(2015)6013)共同资助
